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Embryonic stem cells maintain an undifferentiated state on dendrimer-immobilized surface with D-glucose display
, Article Polymers ; Volume 3, Issue 4 , 2011 , Pages 2078-2087 ; 20734360 (ISSN) ; Kim, M. H ; Kino Oka, M ; Miyazaki, J. I ; Taya, M ; Sharif University of Technology
2011
Abstract
In serial passaging cultures of mouse embryonic stem (ES) cells, we employed a dendrimer-immobilized substrate that displayed D-glucose as a terminal ligand. The D-glucose-displaying dendrimer (GLU/D) surface caused the ES cells to form loosely attached spherical colonies, while those on a gelatin-coated surface formed flatter colonies that were firmly attached to the surface. Despite the morphological similarities between the colonies on the GLU/D surface and aggregates on a conventional bacteriological dish, immunostaining and RT-PCR analyses revealed the maintenance of cells within the spherical colonies on the GLU/D surface in an undifferentiated state with very low expressions of...