Sharif Digital Repository

In tissue engineering, the development of a tissue essentially depends on supply of an adequate amount of nutrients and the design of a proper biophysical micro-environment for cells. The limitation of the available initial number of cells, expensive substances and time consuming experiments are the main bottlenecks in this type of processes. In this regard, mathematical modelling and computational fluid dynamics simulation (CFD) are powerful tools to identify an efficient and optimized design by providing reliable insights of the process. This study presents a mathematical model and CFD simulation of cartilage cell culture under a perfusion flow, which allows not only to characterize the... 

Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs....