Search for: schizosaccharomyces
0.004 seconds

    Quantitative determination of optical trapping strength and viscoelastic moduli inside living cells

    , Article Physical Biology ; Volume 10, Issue 4 , 2013 ; 14783967 (ISSN) Mas, J ; Richardson, A. C ; Reihani, S. N. S ; Oddershede, L. B ; Berg Sorensen, K ; Sharif University of Technology
    With the success of in vitro single-molecule force measurements obtained in recent years, the next step is to perform quantitative force measurements inside a living cell. Optical traps have proven excellent tools for manipulation, also in vivo, where they can be essentially non-invasive under correct wavelength and exposure conditions. It is a pre-requisite for in vivo quantitative force measurements that a precise and reliable force calibration of the tweezers is performed. There are well-established calibration protocols in purely viscous environments; however, as the cellular cytoplasm is viscoelastic, it would be incorrect to use a calibration procedure relying on a viscous environment....