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Multi-Target tracking of human spermatozoa in phase-contrast microscopy image sequences using a hybrid dynamic bayesian network
, Article Scientific Reports ; Volume 8, Issue 1 , 2018 ; 20452322 (ISSN) ; Vosoughi Vahdat, B ; Salman Yazdi, R ; Sharif University of Technology
Nature Publishing Group
2018
Abstract
Male infertility is mostly related to semen and spermatozoa, and any diagnosis or treatment requires the investigation of the motility patterns of spermatozoa. The movements of spermatozoa are fast and involve collision and occlusion with each other. In order to extract the motility patterns of spermatozoa, multi-target tracking (MTT) of spermatozoa is necessary. One of the most important steps of MTT is data association, in which the newly arrived observations are used to update the previous tracks. Dynamic Bayesian network (DBN) is a powerful tool for modeling and solving various types of problems such as tracking and classification. There can also be a hybrid-DBN (HDBN), in which both...
Automatic Analysis and Tracking of Motile Cells in Video Microscopy
, M.Sc. Thesis Sharif University of Technology ; Vosughi Vahdat, Bijan (Supervisor) ; Rabiei, Hamid Reza (Supervisor) ; Salman Yazdi, Reza (Co-Advisor)
Abstract
Analysis of semen and quality assessments of sperm cells is of great importance in scrutiny of male fertility. Several methods have been introduced for analyzing and identification of the sperm motility and morphology in a semen sample. Identifying and tracking of rapid and variant movements of multiple sperms, in a short duration of time, is somewhat difficult and complex for human, even for an expert. Then applying semi-automated or automated (un-supervised) methods, based on image analysis and computing, spread fast and computer aided semen analyzer systems, became wildly used in clinical and research laboratories.
In this paper we propose an efficient multiple tracking methods to...
In this paper we propose an efficient multiple tracking methods to...
Fourier transform infrared spectroscopy: A potential technique for noninvasive detection of spermatogenesis
, Article Avicenna Journal of Medical Biotechnology ; Vol. 6, Issue. 1 , 2014 , pp. 47-52 ; ISSN: 2008-4625 ; Moazeni Pouracil, R. S ; Reza Sadeghi, M ; Sharif University of Technology
Abstract
Background: The seminal plasma is an excellent source for noninvasive detection of spermatogenesis. The seminal plasma of normospermic and azoospermic men has been analyzed for detection of spermatogenesis. Methods: Optical spectroscopy (Attenuated Total Reflectance-Infrared spectroscopy (ATR-IR) and Fourier Transform infrared spectroscopy (FT-IR) has been used to analyze the seminal plasma and the metabolome of seminal plasma for detection of spermatogenesis. Results The seminal plasma of normospermic and azoospermic men has been analyzed by ATR-IR. The results show that there is a pattern variation in the azoospermic men compared to normospermic men. However, the seminal plasma is too...
Metabolomics fingerprinting of the human seminal plasma of asthenozoospermic patients
, Article Molecular Reproduction and Development ; Vol. 81, Issue. 1 , 2014 , pp. 84-86 ; ISSN: 1098-2795 ; Moazeni-Pourasil, R. S ; Jafarzadeh, N ; Savadi-Shiraz, E ; Sharif University of Technology
Abstract
It is estimated that 20% of couples are infertile, and half of these infertility cases are linked to men. One of conditions that can affect male fertility is asthenozoospermia. We applied Raman spectroscopy to the analysis of the metabolome of the human seminal plasma, and used chemometrics on the patterns of Raman spectra obtained. Significant changes were observed in the metabolome of the human seminal plasma of asthenozoospermic patients
Metabolic profiling of seminal plasma from teratozoospermia patients
, Article Journal of Pharmaceutical and Biomedical Analysis ; Volume 178 , January , 2020 ; Chashmniam, S ; Nobakht, F ; Amini, M ; Javidi, A ; Minai Tehrani, A ; Arjmand, B ; Gilany, K ; Sharif University of Technology
Elsevier B. V
2020
Abstract
Teratozoospermia is one of conditions that can cause male infertility. The mechanism of teratozoospermia remains unclear. The knowledge of the metabolites in human seminal plasma (HSP) is meaningful for the pathological study of teratozoospermia. Analysis of changed metabolites in HSP can help understand the cellular mechanism, find the novel biomarkers and subsequently design a diagnosis test. In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in...