Sharif Digital Repository

Protein separation and purification technologies play an essential role in various industries including but not limited to pharmaceuticals, dairy as well as the food sector. Accordingly, a wide variety of techniques such as chromatography and electrophoresis have been developed and utilized extensively over the years for this purpose. Despite their widespread acceptance, conventional techniques still suffer from major limitations and complexities such as short lifetime, low productivity, high-pressure drop and difficulty in scale-up among others. Membrane separation processes have received significant attention in recent years as a promising alternative that can potentially overcome the... 

Re/Mo separation is studied by a new solvent extraction technique which is based on extraction mechanisms of the constituent metals. Based on McCabe-Thiele plots, a flow diagram has been proposed for separation and purification of Re over Mo. Re-recovery section of the diagram shows that Re is extracted when major amount of Mo is removed. For removal of the remained Mo, a two-stage extraction is required at pH = 2 and V a/V o = 1. In order to obtain Re, the resulted aqueous phase is stripped by adding sulfuric acid. According to the results, while more Re is extracted at less pH, optimum extraction of Mo happens at pH = 1.2. Re selectivity over Mo maximizes by approaching of the pH to zero  

The extraction and purification of lipids from the microalgae Chlorella vulgaris have been investigated. First, a mixture of hexane and ethanol was used to extract lipids from the algal biomass. Ultrasonication was employed to disrupt the cell wall and increase the extraction performance. Under these conditions, over 90% of the fatty acids in the biomass were extracted. Second, a biphasic system was formed by adding water and hexane to the extracted crude oil. In this way, fatty acids were mainly distributed in the organic phase (mostly hexane and ethanol) while most of the contaminants remained in the aqueous phase (mostly water and ethanol). Equilibrium distribution data between the phases... 

The simultaneous control of the molecular weight, separation, and purification of polyvinyl acetate was achieved using a cobalt-mediated radical polymerization (CMRP) in a packed column with silica gel particles (PC-CMRP). The controlled radical polymerization of VAc in the packed columns was evaluated from the linear time dependence of Ln[M]0/[M], linear increase of molecular weight with the increase in conversion, and narrow molecular weight distribution. PC-CMRP method was used to produce high-purity polymers with controlled molecular weight and narrow molecular weight distribution without requiring additional purification steps. The high ability of silica gel particles to adsorb free...