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Preparation of biodegradable gelatin/PVA porous scaffolds for skin regeneration
, Article Artificial Cells, Nanomedicine and Biotechnology ; 2016 , Pages 1-8 ; 21691401 (ISSN) ; Dadbin, S ; Frounchi, M ; Rajabi, S ; Sharif University of Technology
Taylor and Francis Ltd
2016
Abstract
Porous scaffolds composed of gelatin/poly (vinyl alcohol), (Gel/PVA), were prepared using combination of freeze gelation and freeze drying methods. The effect of polymer concentration, gelatin/PVA ratio, and glutaraldehyde/gelatin ratio (GA/Gel) was investigated on morphology of pores, swelling ratio, biodegradation, and skin cell culture. At optimum preparation conditions the scaffolds had uniform pore size distributions showing high swelling ratio of 23.6. The scaffolds were of biodegradable nature and almost degraded in 28 days. Human dermal fibroblast cells (HDF) were cultured on the scaffolds and MTS assay was conducted to evaluate the influence of PVA on growth and proliferation of the...
Porous gelatin/poly(ethylene glycol) scaffolds for skin cells
, Article Soft Materials ; Volume 15, Issue 1 , 2017 , Pages 95-102 ; 1539445X (ISSN) ; Frounchi, M ; Dadbin, S ; Sharif University of Technology
Abstract
Biocompatible porous polymeric scaffolds provide a suitable environment for proliferation of stem cells in human body. In this research work, porous gelatin–poly(ethylene glycol), PEG, based scaffolds were prepared using combination of freeze-gelation and freeze-extraction methods. Effects of various parameters such as freezing temperature, cross-linking agent, concentrations of gelatin and PEG and their blending ratio on physical and mechanical properties, swelling ratio, porosity, pore size, and degradation rate of scaffolds were investigated. Also, proliferation of fibroblast skin cells on the scaffolds was examined by MTS assay to assess the suitability of the scaffolds in wound healing...
Preparation of biodegradable gelatin/PVA porous scaffolds for skin regeneration
, Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 45, Issue 5 , 2017 , Pages 928-935 ; 21691401 (ISSN) ; Dadbin, S ; Frounchi, M ; Rajabi, S ; Sharif University of Technology
Taylor and Francis Ltd
2017
Abstract
Porous scaffolds composed of gelatin/poly (vinyl alcohol), (Gel/PVA), were prepared using combination of freeze gelation and freeze drying methods. The effect of polymer concentration, gelatin/PVA ratio, and glutaraldehyde/gelatin ratio (GA/Gel) was investigated on morphology of pores, swelling ratio, biodegradation, and skin cell culture. At optimum preparation conditions the scaffolds had uniform pore size distributions showing high swelling ratio of 23.6. The scaffolds were of biodegradable nature and almost degraded in 28 days. Human dermal fibroblast cells (HDF) were cultured on the scaffolds and MTS assay was conducted to evaluate the influence of PVA on growth and proliferation of the...
Temporary skin grafts based on hybrid graphene oxide-natural biopolymer nanofibers as effective wound healing substitutes: pre-clinical and pathological studies in animal models
, Article Journal of Materials Science: Materials in Medicine ; Volume 28, Issue 5 , 2017 , 73 ; 09574530 (ISSN) ; Eslahi, N ; Mehdipour, A ; Mohammadi, M ; Akbari, M ; Samadikuchaksaraei, A ; Simchi, A ; Sharif University of Technology
Springer New York LLC
2017
Abstract
Abstract: In recent years, temporary skin grafts (TSG) based on natural biopolymers modified with carbon nanostructures have received considerable attention for wound healing. Developments are required to improve physico-mechanical properties of these materials to match to natural skins. Additionally, in-deep pre-clinical examinations are necessary to ensure biological performance and toxicity effect in vivo. In the present work, we show superior acute-wound healing effect of graphene oxide nanosheets embedded in ultrafine biopolymer fibers (60 nm) on adult male rats. Nano-fibrous chitosan-based skin grafts crosslinked by Genepin with physico-mechanical properties close to natural skins were...