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small-rna-sequencing
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MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation
, Article Experimental Eye Research ; Volume 190 , 2020 ; Satarian, L ; Moradi, S ; Sharifi Zarchi, A ; Günther, S ; Kamal, A ; Totonchi, M ; Mowla, S. J ; Braun, T ; Baharvand, H ; Sharif University of Technology
Academic Press
2020
Abstract
Retinal pigment epithelial (RPE) cells are indispensable for eye organogenesis and vision. To realize the therapeutic potential of in vitro-generated RPE cells for cell-replacement therapy of RPE-related retinopathies, molecular mechanisms of RPE specification and maturation need to be investigated. So far, many attempts have been made to decipher the regulatory networks involved in the differentiation of human pluripotent stem cells into RPE cells. Here, we exploited a highly-efficient RPE differentiation protocol to determine global expression patterns of microRNAs (miRNAs) during human embryonic stem cell (hESC) differentiation into RPE using small RNA sequencing. Our results revealed a...
Small RNA sequencing reveals dlk1-dio3 locus-embedded microRNAs as major drivers of ground-state pluripotency
, Article Stem Cell Reports ; Volume 9, Issue 6 , 2017 , Pages 2081-2096 ; 22136711 (ISSN) ; Sharifi Zarchi, A ; Ahmadi, A ; Mollamohammadi, S ; Stubenvoll, A ; Günther, S ; Hosseini Salekdeh, G ; Asgari, S ; Braun, T ; Baharvand, H ; Sharif University of Technology
Abstract
Ground-state pluripotency is a cell state in which pluripotency is established and maintained through efficient repression of endogenous differentiation pathways. Self-renewal and pluripotency of embryonic stem cells (ESCs) are influenced by ESC-associated microRNAs (miRNAs). Here, we provide a comprehensive assessment of the “miRNome” of ESCs cultured under conditions favoring ground-state pluripotency. We found that ground-state ESCs express a distinct set of miRNAs compared with ESCs grown in serum. Interestingly, most “ground-state miRNAs” are encoded by an imprinted region on chromosome 12 within the Dlk1-Dio3 locus. Functional analysis revealed that ground-state miRNAs embedded in the...