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    Design and Manufacturing of a Setup for Solid-state Nanopore Fabrication

    , M.Sc. Thesis Sharif University of Technology Bayat, Alireza (Author) ; Akbari, Javad (Supervisor) ; Taghipour, Mojtaba (Supervisor)
    The aim of the current research is to study the process of making a solidstate nanopore by molding from PDMS polymer and to make a laboratory sample of a solidstate nanopore manufacturing device using this method. Solid-state nanopores are used to control ion transport in microfluidic channels,lab-on-a-chip detection to study nucleic acids and proteins, desalination, nanofluidic transistors,single-cell sensing tools, and particle classification. In general, the smaller the nanopore, the shorter the length and the higher the mechanical stability, the better the performance. In this research, chips have been made on which there is a blade with a sharpness of 10 nanometers and a height of 10... 

    Design and Fabrication of a Microfluidic Chip for Three-dimensional Cancer Cell Culture

    , M.Sc. Thesis Sharif University of Technology Moshksayan, Khashayar (Author) ; Saeedi, Mohammad Saeed (Supervisor) ; Kashaninejad, Navid (Co-Advisor)
    Nowadays, we are witnesses of deaths caused by cancer in large statistical amounts. Vast number of investigations are conducted for anticancer drug evaluation on human and animal cells using traditional two-dimensional and three-dimensional culturing platforms. However, most of them are not successful due to the lack of in-vivo relevant microenvironment in these platforms. One of the best cancer in-vitro cultures is the tumor spheroid which is a spherical cellular aggregation. By culturing these tumor spheroids in a microfluidic chip, it is possible to mimic the in-vivo microenvironment elements such as nutrient and metabolite gradients. Here we present a microfluidic device fabricated using... 

    Cancer Cell Separation Using Spiral Microchannel

    , M.Sc. Thesis Sharif University of Technology Ghadami, Sepehr (Author) ; Saidi, Mohmmad Said (Supervisor) ; Firoozbakhsh, Keykhosrow (Supervisor)
    Particle separation has a variety of applications in biology, chemistry and industry. Among them, circulating tumor cells (CTCs) separation has drawn significant attention to itself due to its high impact on both cancer diagnosis and therapeutics. In recent years, there has been growing interest in using inertial microfluidics to separate micro/nano particles based on their sizes. This technique offers label-free, high throughput, and efficient separation and can be easily fabricated. However, further improvements are needed for potential clinical applications. In this study, a novel inertial separation technique using spiral microchannel with stair-like cross section is introduced. The...