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Spectrophotometric Determination of Glutathione and Cysteine Based on Aggregation of Colloidal Gold Nanoparticles
, M.Sc. Thesis Sharif University of Technology ; Hormozi Nezhad, Mohammad Reza (Supervisor)
Abstract
low-molecular-weigth aminothiols such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play a critical role in many biochemical pathways . Their level in biological fluids such as human plasma and urine are important for clinical diagnostics of a variety of diseases.
We report herein the development of a highly sensitive colorimetric method for detection of cysteine and glutathione using the citrate capped gold nanoparticles (Au-NPs). This thiol containing molecules exhibit intriguing reactivities with Au nanoparticles. The reactivity involves the displacement of the citrate shell by the thiolate shell which is followed by intermolecular electrostatic interactions or...
We report herein the development of a highly sensitive colorimetric method for detection of cysteine and glutathione using the citrate capped gold nanoparticles (Au-NPs). This thiol containing molecules exhibit intriguing reactivities with Au nanoparticles. The reactivity involves the displacement of the citrate shell by the thiolate shell which is followed by intermolecular electrostatic interactions or...
Spectrophotometric Study of Formation of Donor-Acceptor Adducts of Selenium Dioxide with Amines and Pyridine like Bases
, M.Sc. Thesis Sharif University of Technology ; Mohammadi Boghaei, Davar (Supervisor)
Abstract
Donor-acceptor adducts of selenium dioxide were formed with tree aromatic amines, two aliphatic amines and tree pyridine like bases. Characterization of these adducts were done through UV-Vis Spectrophotometer and IR Spectrophotometer and NMR Spectroscopy. In addition, the stoichiometry of these adducts were identified by elemental analysis (CHN). To approve the existence of selenium, Atomic absorption instrument was used.The results obtained from the entioned methods show that the adduct formation between selenium dioxide with amines and pyridine like bases are in following order: p-phenylen diamine.SeO2>NH5.SeO2,en.SeO2,cyclohexylamine.SeO2,...
Spectrophotometric Determination of Ametryn Herbicide in Drainage Water Based on Aggregation of Gold Nanoparticles Capped with Citrate
, M.Sc. Thesis Sharif University of Technology ; Hormozi-Nezhad, Mohammad Reza (Supervisor) ; Torkian, A (Supervisor)
Abstract
Due to vast use of Ametryn herbicide in agricultural and even non-agricultural activities and the possibility of contamination of carp and natural water sources by this herbicide, determination of this herbicide in surface and ground water sources is very important. Therefor introducing simple and sensitive method for determination of Ametryn is critical.
This study demonstrate a simple and sensitive colorimetric method for the detection and determination of Ametryn based on aggregation of citrate capped AuNPs. Studied method relies upon shape and distance dependent AuNPs surface plasmon resonance. By replacing the sulfide containing Ametryn herbicide with citrate molecules on AuNPs...
This study demonstrate a simple and sensitive colorimetric method for the detection and determination of Ametryn based on aggregation of citrate capped AuNPs. Studied method relies upon shape and distance dependent AuNPs surface plasmon resonance. By replacing the sulfide containing Ametryn herbicide with citrate molecules on AuNPs...