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Fabrication and characterization of low-cost, bead-free, durable and hydrophobic electrospun membrane for 3D cell culture
, Article Biomedical Microdevices ; Volume 19, Issue 4 , 2017 ; 13872176 (ISSN) ; Saidi, M. S ; Kashaninejad, N ; Kiyoumarsioskouei, A ; Trung Nguyen, N ; Sharif University of Technology
Abstract
This paper reports the fabrication of electrospun polydimethylsiloxane (PDMS) membranes/scaffolds that are suitable for three-dimensional (3D) cell culture. Through modification the ratio between PDMS and polymethylmethacrylate (PMMA) as carrier polymer, we report the possibility of increasing PDMS weight ratio of up to 6 for electrospinning. Increasing the PDMS content increases the fiber diameter, the pore size, and the hydrophobicity. To our best knowledge, this is the first report describing beads-free, durable and portable electrospun membrane with maximum content of PDMS suitable for cell culture applications. To show the proof-of-concept, we successfully cultured epithelial lung...