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stem-cell-self-renewal
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Controlling differentiation of stem cells for developing personalized organ-on-chip platforms
, Article Advanced Healthcare Materials ; Volume 7, Issue 2 , 2018 ; 21922640 (ISSN) ; Jafari, P ; Sheikh Hassani, M ; Heidary Araghi, B ; Mohammadi, M. H ; Ghafari, A. M ; Hassanpour Tamrin, S ; Pezeshgi Modarres, H ; Rezaei Kolahchi, A ; Ahadian, S ; Sanati Nezhad, A ; Sharif University of Technology
Wiley-VCH Verlag
2018
Abstract
Organ-on-chip (OOC) platforms have attracted attentions of pharmaceutical companies as powerful tools for screening of existing drugs and development of new drug candidates. OOCs have primarily used human cell lines or primary cells to develop biomimetic tissue models. However, the ability of human stem cells in unlimited self-renewal and differentiation into multiple lineages has made them attractive for OOCs. The microfluidic technology has enabled precise control of stem cell differentiation using soluble factors, biophysical cues, and electromagnetic signals. This study discusses different tissue- and organ-on-chip platforms (i.e., skin, brain, blood–brain barrier, bone marrow, heart,...
Small RNA sequencing reveals dlk1-dio3 locus-embedded microRNAs as major drivers of ground-state pluripotency
, Article Stem Cell Reports ; Volume 9, Issue 6 , 2017 , Pages 2081-2096 ; 22136711 (ISSN) ; Sharifi Zarchi, A ; Ahmadi, A ; Mollamohammadi, S ; Stubenvoll, A ; Günther, S ; Hosseini Salekdeh, G ; Asgari, S ; Braun, T ; Baharvand, H ; Sharif University of Technology
Abstract
Ground-state pluripotency is a cell state in which pluripotency is established and maintained through efficient repression of endogenous differentiation pathways. Self-renewal and pluripotency of embryonic stem cells (ESCs) are influenced by ESC-associated microRNAs (miRNAs). Here, we provide a comprehensive assessment of the “miRNome” of ESCs cultured under conditions favoring ground-state pluripotency. We found that ground-state ESCs express a distinct set of miRNAs compared with ESCs grown in serum. Interestingly, most “ground-state miRNAs” are encoded by an imprinted region on chromosome 12 within the Dlk1-Dio3 locus. Functional analysis revealed that ground-state miRNAs embedded in the...