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survival-analysis
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MiR-361-5p as a promising qRT-PCR internal control for tumor and normal breast tissues
, Article PLoS ONE ; Volume 16, Issue 6 June , 2021 ; 19326203 (ISSN) ; Salimi, A ; Rahmani, S ; Nafissi, N ; Sharifi Zarchi, A ; Mowla, S. J ; Sharif University of Technology
Public Library of Science
2021
Abstract
Background: One of the most widely used evaluation methods in miRNA experiments is qRT-PCR. However, selecting suitable internal controls (IC) is crucial for qRT-PCR experiments. Currently, there is no consensus on the ICs for miRNA qRT-PCR experiments in breast cancer. To this end, we tried to identify the most stable (the least expression alteration) and promising miRNAs in normal and tumor breast tissues by employing TCGA miRNA-Seq data and then experimentally validated them on fresh clinical samples. Methods: A multi-component scoring system was used which takes into account multiple expression stability criteria as well as correlation with clinical characteristics. Furthermore, we...
Cytochrome 4z1 expression is correlated with poor prognosis in patients with cervical cancer
, Article Current Oncology ; Volume 28, Issue 5 , 2021 , Pages 3573-3584 ; 17187729 (ISSN) ; Alshammari, F. O. F. O ; Youssef, A. M. M ; Al Sarayra, Y. M ; Al Saraireh, R. A ; Al Muhaisen, G. H ; Al Mahdy, Y. S ; Al Kharabsheh, A. M ; Abufraijeh, S. M ; Al Rawashdeh, H. M ; Sharif University of Technology
MDPI
2021
Abstract
Background: cervical cancer is one of the most common malignancies in women worldwide and its management remains challenging and complex. As Cytochrome4Z1 (CYP4Z1) is overex-pressed in many tumours, its expression in cervical cancer is unknown. Therefore, the present study aimed to evaluate CYP4Z1 expression in cervical cancers. Methods: CYP4Z1 expression was immunohistochemically assessed in 100 cases of cervical cancers along with ten normal cervix tissues, and the enzyme’s relationship to several clinicopathological features and survival was explored. Results: CYP4Z1 was strongly expressed in 55% of cervical cancer patients. Normal cervix samples were negative for CYP4Z1 expression....
An integrated analysis to predict micro-RNAs targeting both stemness and metastasis in breast cancer stem cells
, Article Journal of Cellular and Molecular Medicine ; Volume 23, Issue 4 , 2019 , Pages 2442-2456 ; 15821838 (ISSN) ; Sharifi Zarchi, A ; Firouzi, J ; Azimi, M ; Zarghami, N ; Alizadeh, E ; Ebrahimi, M ; Sharif University of Technology
Blackwell Publishing Inc
2019
Abstract
Several evidences support the idea that a small population of tumour cells representing self-renewal potential are involved in initiation, maintenance, metastasis, and outcomes of cancer therapy. Elucidation of microRNAs/genes regulatory networks activated in cancer stem cells (CSCs) is necessary for the identification of new targets for cancer therapy. The aim of the present study was to predict the miRNAs pattern, which can target both metastasis and self-renewal pathways using integration of literature and data mining. For this purpose, mammospheres derived from MCF-7, MDA-MB231, and MDA-MB468 were used as breast CSCs model. They had higher migration, invasion, and colony formation...