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    A 32kb 90nm 10T-cell sub-threshold SRAM with improved read and write SNM

    , Article 2013 21st Iranian Conference on Electrical Engineering ; May , 2013 ; 9781467356343 (ISBN) Hassanzadeh, S ; Zamani, M ; Hajsadeghi, K ; Sharif University of Technology
    The constraints of power saving have compelled SRAM designers to consider sub-threshold area as a viable choice. The biggest barrier of this progress is the stability of SRAM's cells and the correct operations. In this paper a 10T cell structure has been proposed with 90% read and 50% write SNM improvement in comparison to the conventional 6T cell. The hold SNM value is about the 6T cell SRAM. Also using differential read method in the proposed structure causes high read performance and using simpler sense amplifier. The symmetric configuration of this structure helps the SRAM has simpler layout and lower transistor mismatch. Using 90nm TSMC CMOS, 32kb 10T cell SRAM in sub-threshold area is... 

    Theoretical modeling of actin-retrograde-flow passing clusters of confined T cell receptors

    , Article Mathematical Biosciences ; Volume 283 , 2017 , Pages 1-6 ; 00255564 (ISSN) Ghasemi V., A ; Firoozabadi, B ; Saidi, M. S ; Sharif University of Technology
    Elsevier Inc  2017
    Through the activation process of T cells, actin filaments move from the cell periphery toward the cell center. The moving filaments engage with T cell receptors and thus contribute to transportation of the signaling molecules. To study the connection between the moving actin filaments and T cell receptors, an experiment available in the literature has measured filaments flow velocity passing over a region of confined clusters of receptors. It shows that flow velocity decreases in the proximity of the receptors, and then regains its normal value after traversing the region, suggesting a dissipative friction-like connection. In this work, we develop a minimal theoretical model to re-examine... 

    A novel low power 8T-cell sub-threshold SRAM with improved read-SNM

    , Article Proceedings of the 2013 8th International Conference on Design and Technology of Integrated Systems in Nanoscale Era, DTIS 2013 ; 2013 , Pages 35-38 ; 9781467360388 (ISBN) Hassanzadeh, S ; Zamani, M ; Hajsadeghi, K ; Saeidi, R ; Sharif University of Technology
    The fast growth of battery-operated portable applications has compelled the static random access memory (SRAM) designers to consider sub-threshold operation as a viable choice to reduce the power consumption. To increase the hold, read and write static noise margin (SNM) in the sub-threshold regime many structures has been proposed adding extra transistors to the conventional 6T-cell. In this paper we propose a new 8T-cell SRAM that shows 90% improvement in read SNM while write and hold SNM reduction can be ignored (this negligible reduction is due to the two stack transistors in the proposed 8T-cell). Benefiting differential output voltage in the read operation, sense amplifier design is... 

    A 32kb 90nm 9T-cell sub-threshold SRAM with improved read and write SNM

    , Article Proceedings of the 2013 8th International Conference on Design and Technology of Integrated Systems in Nanoscale Era, DTIS 2013 ; 2013 , Pages 104-107 ; 9781467360388 (ISBN) Zamani, M ; Hassanzadeh, S ; Hajsadeghi, K ; Saeidi, R ; Sharif University of Technology
    The fast growth of battery operated devices has made low power SRAM designs a necessity in recent years. Moreover, embedded SRAM units have become an important block in modern SoCs. The SRAM performance is limited by the cell stability during different operation. By adding extra transistor to the conventional 6T-cell, hold, read and write static noise margin (SNM) can be improved in the sub-threshold SRAM. In this paper we proposed a new 9T-cell SRAM that shows 80% and 50% improvement in read and write SNM respectively in comparison to the conventional 6T-cell SRAM. Using stack transistors in the leakage current path, the new structure shows lower bitline leakage assisting the sense... 

    CRISPR-Cas, a robust gene-editing technology in the era of modern cancer immunotherapy

    , Article Cancer Cell International ; Volume 20, Issue 1 , September , 2020 Miri, S. M ; Tafsiri, E ; Cho, W. C. S ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Cancer immunotherapy has been emerged as a promising strategy for treatment of a broad spectrum of malignancies ranging from hematological to solid tumors. One of the principal approaches of cancer immunotherapy is transfer of natural or engineered tumor-specific T-cells into patients, a so called "adoptive cell transfer", or ACT, process. Construction of allogeneic T-cells is dependent on the employment of a gene-editing tool to modify donor-extracted T-cells and prepare them to specifically act against tumor cells with enhanced function and durability and least side-effects. In this context, CRISPR technology can be used to produce universal T-cells, equipped with recombinant T cell... 

    CRISPRi-mediated knock-down of PRDM1/BLIMP1 programs central memory differentiation in ex vivo-expanded human T cells

    , Article BioImpacts ; Volume 12, Issue 4 , 2022 , Pages 337-347 ; 22285652 (ISSN) Azadbakht, M ; Sayadmanesh, A ; Nazer, N ; Ahmadi, A ; Hemmati, S ; Mohammadzade, H ; Ebrahimi, M ; Baharvand, H ; Khalaj, B ; Aghamaali, M. R ; Basiri, M ; Sharif University of Technology
    Tabriz University of Medical Sciences  2022
    Introduction: B lymphocyte-induced maturation protein 1 (BLIMP1) encoded by the positive regulatory domain 1 gene (PRDM1), is a key regulator in T cell differentiation in mouse models. BLIMP1-deficiency results in a lower effector phenotype and a higher memory phenotype. Methods: In this study, we aimed to determine the role of transcription factor BLIMP1 in human T cell differentiation. Specifically, we investigated the role of BLIMP1 in memory differentiation and exhaustion of human T cells. We used CRISPR interference (CRISPRi) to knock-down BLIMP1 and investigated the differential expressions of T cell memory and exhaustion markers in BLIMP1-deficient T cells in comparison with... 

    A Boolean network control algorithm guided by forward dynamic programming

    , Article PLoS ONE ; Volume 14, Issue 5 , 2019 ; 19326203 (ISSN) Moradi, M ; Goliaei, S ; Foroughmand Araabi, M. H ; Sharif University of Technology
    Public Library of Science  2019
    Control problem in a biological system is the problem of finding an interventional policy for changing the state of the biological system from an undesirable state, e.g. disease, into a desirable healthy state. Boolean networks are utilized as a mathematical model for gene regulatory networks. This paper provides an algorithm to solve the control problem in Boolean networks. The proposed algorithm is implemented and applied on two biological systems: T-cell receptor network and Drosophila melanogaster network. Results show that the proposed algorithm works faster in solving the control problem over these networks, while having similar accuracy, in comparison to previous exact methods. Source... 

    Genome-wide DNA methylation profiling in ectopic and eutopic of endometrial tissues

    , Article Journal of Assisted Reproduction and Genetics ; Volume 36, Issue 8 , 2019 , Pages 1743-1752 ; 10580468 (ISSN) Barjaste, N ; Shahhoseini, M ; Afsharian, P ; Sharifi Zarchi, A ; Masoudi Nejad, A ; Sharif University of Technology
    Springer New York LLC  2019
    Purpose: Endometriosis is a gynecological disease that causes the uterine lining to appear in other organs outside the uterus. As DNA methylation has an important role in this disorder, its profiling can reveal new information to improve the diagnosis and treatment of endometriosis patients. Methods: We conducted a genome-wide methylation profiling of ectopic and eutopic endometrial tissues from women with and without endometriosis using Infinium Human Methylation 450K BeadChip arrays. DNA methylation samples were collected from nine ectopic and nine eutopic endometrial tissues of endometriosis and six endometrial tissues of healthy controls. Results: Correlation heatmaps and the principal... 

    Oncolytic newcastle disease virus delivered by mesenchymal stem cells-engineered system enhances the therapeutic effects altering tumor microenvironment

    , Article Virology Journal ; Volume 17, Issue 1 , 2020 Keshavarz, M ; Ebrahimzadeh, M. S ; Miri, S. M ; Dianat Moghadam, H ; Ghorbanhosseini, S. S ; Mohebbi, S. R ; Keyvani, H ; Ghaemi, A ; Sharif University of Technology
    BioMed Central Ltd  2020
    Background: Human papillomavirus (HPV)-associated malignancy remain a main cause of cancer in men and women. Cancer immunotherapy has represented great potential as a new promising cancer therapeutic approach. Here, we report Mesenchymal stem cells (MSCs) as a carrier for the delivery of oncolytic Newcastle disease virus (NDV) for the treatment of HPV-associated tumor. Methods: For this purpose, MSCs obtained from the bone marrow of C57BL mice, then cultured and characterized subsequently by the flow cytometry analysis for the presence of cell surface markers. In this study, we sought out to determine the impacts of MSCs loaded with oncolytic NDV on splenic T cell and cytokine immune... 

    Distinct dynamics of migratory response to pd-1 and ctla-4 blockade reveals new mechanistic insights for potential t-cell reinvigoration following immune checkpoint blockade

    , Article Cells ; Volume 11, Issue 22 , 2022 ; 20734409 (ISSN) Safaeifard, F ; Goliaei, B ; Aref, A. R ; Foroughmand-Araabi, M. H ; Goliaei, S ; Lorch, J ; Jenkins, R. W ; Barbie, D. A ; Shariatpanahi, S. P ; Rüegg, C ; Sharif University of Technology
    MDPI  2022
    Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death protein 1 (PD-1), two clinically relevant targets for the immunotherapy of cancer, are negative regulators of T-cell activation and migration. Optimizing the therapeutic response to CTLA-4 and PD-1 blockade calls for a more comprehensive insight into the coordinated function of these immune regulators. Mathematical modeling can be used to elucidate nonlinear tumor–immune interactions and highlight the underlying mechanisms to tackle the problem. Here, we investigated and statistically characterized the dynamics of T-cell migration as a measure of the functional response to these pathways. We used a previously...