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    Biodesulfurization of dibenzothiophene by a newly isolated thermophilic bacteria strain

    , Article Iranian Journal of Chemistry and Chemical Engineering ; Volume 25, Issue 3 , 2006 , Pages 65-72 ; 10219986 (ISSN) Hosseini, S. A ; Yaghmaei, S ; Mousavi, S.M ; Jadidi, A.R ; Sharif University of Technology
    2006
    Abstract
    Microbiological analyses of soil chronically exposed to petroleum complex compounds of some oil springs in south of Iran resulted in isolation and purification of a new native thermophilic strain which is capable to desulfurize petroleum sulfur compounds by 4-S mechanism. Dibenzothiophene (DBT) was selected as a complex sulfiir compound model and many experiments were done to identify the metabolic pathway. The results of these experiments show that DBT is ultimately converted to 2-hydroxybiphenyl (2-HBP) and sulfite. This is a special metabolic pathway in that there is no effect on the carbon skeleton of organic compounds and would be ideal for desulfurization to upgrade the petroleum... 

    Efficiency of copper bioleaching of two mesophilic and thermophilic bacteria isolated from chalcopyrite concentrate of Kerman-Yazd regions in Iran

    , Article Scientia Iranica ; Volume 14, Issue 2 , 2007 , Pages 180-184 ; 10263098 (ISSN) Mousavi, S. M ; Yaghmaei, S ; Vossoughi, M ; Jafari, A ; Sharif University of Technology
    Sharif University of Technology  2007
    Abstract
    A mesophilic iron oxidizing bacterium, Acidithiobacillus ferrooxidans, has been isolated (33°C) from a typical chalcopyrite copper concentrate of the Sarcheshmeh Copper Mine in the region of Kerman located in the south of Iran. In addition, a thermophilic iron oxidizing bacterium, Sulfobacillus spp., has been isolated (60°C) from the Kooshk Lead and Zinc Mine near the city of Yazd in the mid-west region of Iran. The variation of pH, ferrous and ferric concentration and cell growth on the time and effects of some factors, such as temperature and initial ferrous concentration, on the bioleaching of chalcopyrite concentrate obtained from Sarcheshmeh Copper Mine, were investigated. Bioleaching... 

    Empirical Study for Removal of Oil Contamination by Thermophilic Bacteria

    , M.Sc. Thesis Sharif University of Technology Babaeivelni, Kamel (Author) ; Vossoughi, Manuchehr (Supervisor) ; Alemzade, Iran (Supervisor)
    Abstract
    Biodegradation has always been as a proper method for treatment of oil contamination. Accordingly, several investigations have been done by mesophilic bacteria for biodegradation of hydrocarbons while few studies have been conducted about thermophilic bacteria.
    There are many factors like high growth rate of thermophilic bacteria and good resistance of enzymes secreted from thermophiles against physical and chemical variations lead to the extensive use of thermophilic bacteria for removal of oil contamination. The important factors should be optimized in order to choose a suitable method for the removal of oil contaminations. Optimal conditions for oil contamination removal by... 

    Empirical Studies and Modeling a Solid-State Fermentation Bioreactor In order to Treat Hydrocarbon Contaminated Soil

    , M.Sc. Thesis Sharif University of Technology Azizi, Yousof (Author) ; Vossoughi, Manoochehr (Supervisor) ; Safekordi, Ali Akbar (Supervisor)
    Abstract
    One of the main environmental problems in the world particularly in Iran is the entrance of oil pollutions from petroleum waste and its related industries into the soil. There are various methods to eliminate petroleum pollutions from the environment such as physical, chemical, thermal and biological methods. In biological remediation, the environmental pollutions are eliminated by means of plants and microorganisms. Over the last two decades, ioremediation have been studied widely by experts of various fields. One of the most advantages of bioremediation comparing with the other methods is its low cost and the ability to perform at the site (in situ).In this project, we extracted two kinds... 

    Study of Biological Extraction Kinetics of Heavy Metals from Spent Catalyst by Thermophilic Bacteria

    , M.Sc. Thesis Sharif University of Technology Gerayeli, Faezeh (Author) ; Yaghmaei, Soheyla (Supervisor) ; Mousavi, Mohammad (Supervisor)
    Abstract
    Every year, an enormous amount of catalysts are consumed in oil refineries during refining processes. After some regeneration cycles in order to recover their activity, they are not anymore effective and must be disposed. These spent catalysts are regarded as dangerous solid wastes as they significantly include some contaminations like vanadium, nickel, arsenic, sodium. From another point of view, the metals present in spent catalysts are highly valuable and are used extensively in steel industry and manufacture of special alloys. So, it sounds profitable to extract and recover these metals from such a cheap source instead of minerals and ores. Basically, there are two main processes, hydro-... 

    Bacterial leaching of low-grade ZnS concentrate using indigenous mesophilic and thermophilic strains

    , Article Hydrometallurgy ; Volume 85, Issue 1 , 2007 , Pages 59-65 ; 0304386X (ISSN) Mousavi, S. M ; Yaghmaei, S ; Vossoughi, M ; Jafari, A ; Roostaazad, R ; Turunen, I ; Sharif University of Technology
    2007
    Abstract
    In this study low-grade sphalerite has been treated by the bioleaching process using native cultures of Acidithiobacillus ferrooxidans and Sulfobacillus thermosulfido-oxidans in order to determine the ability of these bacteria to the leaching of zinc. The effects of bacterial strain, pH, temperature, pulp density, iron precipitation, and initial concentration of ferric iron on the zinc leaching were evaluated. The bioleaching experiments were carried out in shake flasks at pH 1.5, 180 rpm, 33 °C and 60 °C for mesophilic and thermophilic bacteria, respectively. Compared with the use of laboratory reference strains or control conditions, zinc recovery from the respective concentrate was... 

    Naphthalene metabolism in Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic microorganism

    , Article Chemosphere ; Volume 72, Issue 6 , 2008 , Pages 905-909 ; 00456535 (ISSN) Zeinali, M ; Vossoughi, M ; Ardestani, S. K ; Sharif University of Technology
    2008
    Abstract
    The thermophilic bacterium Nocardia otitidiscaviarum strain TSH1, originally isolated in our laboratory from a petroindustrial wastewater contaminated soil in Iran, grows at 50 °C on a broad range of hydrocarbons. Transformation of naphthalene by strain TSH1 which is able to use this two ring-polycyclic aromatic hydrocarbon (PAH) as a sole source of carbon and energy was investigated. The metabolic pathway was elucidated by identifying metabolites, biotransformation studies and monitoring enzyme activities in cell-free extracts. The identification of metabolites suggests that strain TSH1 initiates its attack on naphthalene by dioxygenation at its C-1 and C-2 positions to give... 

    Experimental Study and Optimization of Bioremediation of PAHs by Newly Isolated Thermophilic Filamentous Fungi in Slurry Bioreactor

    , M.Sc. Thesis Sharif University of Technology Sarayloo, Ehsan (Author) ; Vossoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Abstract
    Sustainable development requires the development and promotion of environmental management and a constant search for green technologies to treat a wide range of aquatic and terrestrial habitats contaminated by increasing anthropogenic activities. In this work we investigated the ability of four previously isolated fungi, BBRC 9057, BBRC 20020, BBRC 9058 and BBRC 9002, in degradation of polycyclic (PAHs) and the effect of UV irritation on fungi cell growth and degradation of PAHs. Also we studied the degradation of anthracene by thermophilic filamentous fungi in liquid and soil slurry bioreactor. Cell growth and anthracene degradation ability of BBRC 9057 increased 6.51 and 1.82 times rather... 

    Thermophilic Anaerobic Digestion of Organic Fraction of Municipal Solid Waste

    , M.Sc. Thesis Sharif University of Technology Fahim, Farshad (Author) ; Shaygan Salek, Jalaloddin (Supervisor)
    Abstract
    Due to the large portion of organic fraction of municipal solid wastes in Iran, the needing for stabilization and treatment of this waste is obvious. An appropriate method for treatment of these wastes is anaerobic digestion. By skimming through open literature which are investigated about anaerobic bioreactors, declared that despite the benefits of thermophilic bioreactors over mesophilic ones, (more destruction of pathogens, more removal of volatile solids, more production of biogas and greater resilient to organic loading rate shocks) but because of more difficulties in controlling of thermophilic bioreactors, most studies have been done in mesophilic state. This motivated us to a deeper... 

    Experimental Removal of Aromatic Hydrocarbons by Thermophilic Bacteria in a Fixed Bed Reactor

    , M.Sc. Thesis Sharif University of Technology Bokaee, Shima (Author) ; Borghei, Mehdi (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Zeinali, Majid (Supervisor)
    Abstract
    According to the environmental lows and standards, wastewater of petroleum industries such as refineries and petrochemical that include many petroleum compounds like aromatic and aliphatic hydrocarbons, must be treated up to desirable limit, before entering the collecting network. Using microorganisms is one of the fundamental ways of biodegradation of these polluted compounds. Since phenolic compounds are the main components of raw petroleum, strains that are capable of tolerating phenol and usage of this poisonous hydrocarbon are specifically important. Therefore, in this study the potentiality of the indigenous thermophilic bacterium Nocardia otitidiscaviarum strain TSH1, for degradation... 

    Thermal Simulation of Bioheap

    , Ph.D. Dissertation Sharif University of Technology Mahmoudian, Alireza (Author) ; Sadrnezhad, Khatiboleslam (Supervisor)
    Abstract
    Nowadays, because of drastically decrease of copper sulfide ore’s grade, and difficulties in leaching of them, new processes of copper production like bioleaching technique become significant. Finding the optimal conditions for bioleaching, require investing a lot of time and cost. Computer simulation is useful in this case. Temperature, affects the kinetics of dissolution reactions, type and growth rate of bacteria, and finally, rate and amount of recovery of valuable copper metal from ores, so determination of temperature within the bioheap is very important. Thermophiles are better than mesophiles in chalcopyrite bioleaching and in the range of 55 to 65 degree centigrade, thermophiles... 

    Study of a newly isolated thermophilic bacterium capable of Kuhemond heavy crude oil and dibenzothiophene biodesulfurization following 4S pathway at 60°C

    , Article Journal of Chemical Technology and Biotechnology ; Volume 83, Issue 12 , June , 2008 , Pages 1689-1693 ; 02682575 (ISSN) Torkamani, S ; Shayegan, J ; Yaghmaei, S ; Alemzadeh, I ; Sharif University of Technology
    2008
    Abstract
    Background: To meet stringent emission standards stipulated by regulatory agencies, the oil industry is required to bring down the sulfur content in fuels. As some compounds cannot be desulfurized by existing desulfurizing processes (such as hydrodesulfurization, HDS) biodesulfurization has become an interesting topic for researchers. Most of the isolated biodesulfurizing microorganisms are capable of desulfurization of refined products whose predominant sulfur species are dibenzothiophenes so biocatalyst development is still needed to desulfurize the spectrum of sulfur-bearing compounds present in whole crude. Results: The first desulfurizing bacterium active at 60 °C has been isolated,... 

    Experimental Study the Treatment of Oily Wastewater by Thermophilic External Membrane Bioreactor(MBR)

    , M.Sc. Thesis Sharif University of Technology Geramiraz, Farzaneh (Author) ; Kariminia, Hamidreza (Supervisor) ; Vosoughi, Manouchehr (Supervisor) ; Hesampour, Mehrdad (Co-Advisor)
    Abstract
    The membrane bioreactor (MBR) as an alternative method for simultaneous biodegradation and separation of oily water has received more attention, recently. Normally the MBR works at ambient temperature; therefore, the yield of biodegradation recalcitrant components is low. Increasing temperature improves the efficiency of degradation and reduces the amount of produced sludge. In this study the performance of an aerobic thermophilic and mesophilic membrane bioreactor (MBR) for treating oily wastewater has been investigated. In batch conditions (without applying the membrane), a comparison between COD removal in mesophilic and thermophilic conditions was done. In thermophilic conditions COD and... 

    Screening and optimization of effective parameters in biological extraction of heavy metals from refinery spent catalysts using a thermophilic bacterium

    , Article Separation and Purification Technology ; Volume 118 , October , 2013 , Pages 151-161 ; 13835866 (ISSN) Gerayeli, F ; Ghojavand, F ; Mousavi, S. M ; Yaghmaei, S ; Amiri, F ; Sharif University of Technology
    2013
    Abstract
    This research aimed at finding the factors with more impact on the bioleaching of a spent hydrocracking catalyst by thermophilic acidophilic archaea called Acidianus brierleyi and determining the optimal condition in order to reach the maximum metals recovery. Initially, four more significant factors affecting the bioleaching efficiency including pH, pulp density, inoculation and elemental sulfur concentration, were screened and identified among ten key factors using Plackett-Burman factorial design. Next, a central composite design was applied to obtain the optimum conditions for achieving the maximum efficiency of the bioleaching process. pH 1.6, pulp density 0.6% (w/v), inoculation 4%... 

    The immunomodulatory effects of probiotics on respiratory viral infections: A hint for COVID-19 treatment?

    , Article Microbial Pathogenesis ; Volume 148 , November , 2020 Mahooti, M ; Miri, S. M ; Abdolalipour, E ; Ghaemi, A ; Sharif University of Technology
    Academic Press  2020
    Abstract
    Respiratory virus infections are among the most prevalent diseases in humans and contribute to morbidity and mortality in all age groups. Moreover, since they can evolve fast and cross the species barrier, some of these viruses, such as influenza A and coronaviruses, have sometimes caused epidemics or pandemics and were associated with more serious clinical diseases and even mortality. The recently identified Coronavirus Disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a Public Health Emergency of International concern and has been associated with rapidly progressive pneumonia. To ensure protection against emerging respiratory tract... 

    Characterization of a moderate thermophilic Nocardia species able to grow on polycyclic aromatic hydrocarbons

    , Article Letters in Applied Microbiology ; Volume 45, Issue 6 , December , 2007 , Pages 622-628 ; 02668254 (ISSN) Zeinali, M ; Vossoughi, M ; Ardestani, S. K ; Sharif University of Technology
    2007
    Abstract
    Aims: Our goal was the characterization of a new moderate thermophilic polycyclic aromatic hydrocarbon (PAH)-utilizing Nocardia strain. Methods and Results: A thermophilic bacterium, strain TSH1, was isolated from a contaminated soil. The macroscopic and microscopic features fit well with the description of Nocardia species. The results of 16S rRNA gene analysis showed 100% match to the type strain of N. otitidiscaviarum DSM 43242T. Strain TSH1 showed the same mycolic acid pattern as the type strain of N. otitidiscaviarum but its fatty acid profile did not permit identification to the species level. The carbon utilization profile of strain TSH1 was different from N. otitidiscaviarum. The... 

    Degradation of phenanthrene and anthracene by Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic bacterium

    , Article Journal of Applied Microbiology ; Volume 105, Issue 2 , 11 July , 2008 , Pages 398-406 ; 13645072 (ISSN) Zeinali, M ; Vossoughi, M ; Ardestani, S. K ; Sharif University of Technology
    2008
    Abstract
    Aims: The metabolism of phenanthrene and anthracene by a moderate thermophilic Nocardia otitidiscaviarum strain TSH1 was examined. Methods and Results: When strain TSH1 was grown in the presence of anthracene, four metabolites were identified as 1,2-dihydroxy-1,2-dihydroanthracene, 3-(2-carboxyvinyl)naphthalene-2-carboxylic acid, 2,3-dihydroxynaphthalene and benzoic acid using gas chromatography-mass spectrometry (GC-MS), reverse phase-high performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC). Degradation studies with phenanthrene revealed 2,2′-diphenic acid, phthalic acid, 4-hydroxyphenylacetic acid, o-hydroxyphenylacetic acid, benzoic acid, a phenanthrene...