Search for: three-temperature
Designing a polymerase chain reaction device working with radiation and convection heat transfer, Article 2017 International Conference on Nanomaterials and Biomaterials, ICNB 2017, 11 December 2017 through 13 December 2017 ; Volume 350, Issue 1 , 2018 ; 17578981 (ISSN) ; Kalan, K ; Shamloo, A ; Sharif University of Technology
Institute of Physics Publishing 2018
Gene proliferation is vital for infectious and genetic diseases diagnosis from a blood sample, even before birth. In addition, DNA sequencing, genetic finger-print analyzing, and genetic mutation detecting can be mentioned as other procedures requiring gene reproduction. Polymerase chain reaction, briefly known as PCR, is a convenient and effective way to accomplish this task; where the DNA containing sample faces three temperature phases alternatively. These phases are known as denaturation, annealing, and elongation/extension which in this study -regarding the type of the primers and the target DNA sequence- are set to occur at 95, 58, and 72 degrees of Celsius. In this study, a PCR device...
Theoretical analysis of polymeric and crystalline thick films melting with a single gold nanoparticle embedded in a transparent matrix under nanosecond pulsed laser excitation, Article Journal of Physics D: Applied Physics ; Volume 45, Issue 47 , 2012 ; 00223727 (ISSN) ; Bahrampour, A. R ; Pepe, G. P ; Sharif University of Technology
Optothermal properties of noble metal nanoparticles can be used in a wide range of applications. This paper presents the results of a theoretical study on the utilization of laser-induced heating of a gold nanoparticle (GNP) to melt a region of a transparent material with sub-wavelength spatial resolution. The considered system consists of a 10 or 15nm diameter GNP fixed inside a silica substrate. The silica surface is covered with a thick film of the transparent polymeric or crystalline material. The heating and melting processes are studied under a 7.5ns pulsed laser illumination. Calculations are conducted under three temperature limits, on the maximum temperature of the free electrons,...
Partitioning of penicillin G acylase in aqueous two-phase systems of poly(ethylene glycol) 20000 or 35000 and potassium dihydrogen phosphate or sodium citrate, Article Journal of Chemical and Engineering Data ; Volume 55, Issue 1 , 2010 , Pages 243-248 ; 00219568 (ISSN) ; Vossoughi, M ; Taghikhani, V ; Sharif University of Technology
The partitioning of penicillin G acylase in aqueous two-phase systems (ATPS's) containing poly(ethylene glycol) (PEG) 20000 or 35000 and potassium dihydrogen phosphate (KH2PO4) or sodium citrate (C 6H5Na3O7 ·5H2O) has been measured at three temperatures, (301.2, 307.2, and 310.2) K. The effects of temperature, polymer molecular weight, and polymer and salt concentrations on the partitioning of penicillin G in the ATPS were studied. The experimental data showed that the composition of salt has a large effect on partitioning of penicillin G in ATPS, and the temperature of the system has a small effect on the partitioning. The UNIFAC-FV group contribution model (Pazuki et al., Ind. Eng. Chem....