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    High-throughput, label-free isolation of white blood cells from whole blood using parallel spiral microchannels with u-shaped cross-section

    , Article Biosensors ; Volume 11, Issue 11 , 2021 ; 20796374 (ISSN) Mehran, A ; Rostami, P ; Saidi, M. S ; Firoozabadi, B ; Kashaninejad, N ; Sharif University of Technology
    MDPI  2021
    Abstract
    Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner... 

    Bilayered heparinized vascular graft fabricated by combining electrospinning and freeze drying methods

    , Article Materials Science and Engineering C ; Volume 94 , 2019 , Pages 1067-1076 ; 09284931 (ISSN) Khayat Norouzi, S ; Shamloo, A ; Sharif University of Technology
    Elsevier Ltd  2019
    Abstract
    Small diameter vascular grafts (<6 mm) are highly demanded for patients suffering from severe occluded arteries to be used as a bypass or substituted conduit. Fabricating a graft with appropriate structural, mechanical and cell growth properties which has simultaneously anti-thrombogenic trait is a challenge nowadays. Here, we proposed a bilayer heparinized vascular graft that can mimic the structural and mechanical characteristics close to those of the native coronary artery by combining electrospinning and freeze drying methods. In this study, the inner layer was made by co-electrospinning of synthetic polymer, poly-caprolactone (PCL) and the natural polymer, gelatin (Gel). Also, heparin... 

    Bilayered heparinized vascular graft fabricated by combining electrospinning and freeze drying methods

    , Article Materials Science and Engineering C ; Volume 94 , 2019 , Pages 1067-1076 ; 09284931 (ISSN) Khayat Norouzi, S ; Shamloo, A ; Sharif University of Technology
    Elsevier Ltd  2019
    Abstract
    Small diameter vascular grafts (<6 mm) are highly demanded for patients suffering from severe occluded arteries to be used as a bypass or substituted conduit. Fabricating a graft with appropriate structural, mechanical and cell growth properties which has simultaneously anti-thrombogenic trait is a challenge nowadays. Here, we proposed a bilayer heparinized vascular graft that can mimic the structural and mechanical characteristics close to those of the native coronary artery by combining electrospinning and freeze drying methods. In this study, the inner layer was made by co-electrospinning of synthetic polymer, poly-caprolactone (PCL) and the natural polymer, gelatin (Gel). Also, heparin... 

    Investigation of a two-step device implementing magnetophoresis and dielectrophoresis for separation of circulating tumor cells from blood cells

    , Article Engineering in Life Sciences ; Volume 20, Issue 7 , 2020 , Pages 296-304 Shamloo, A ; Yazdani, A ; Saghafifar, F ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    Identifying tumor cells from a pool of other cells has always been an appealing topic for different purposes. The objective of this study is to discriminate circulating tumor cells (CTCs) from blood cells for diagnostic purposes in a novel microfluidic device using two active methods: magnetophoresis and dielectrophoresis. The most specific feature of this device is the differentiation of CTCs without labeling them in order to achieve a more reliable and less complicated method. This device was analyzed and evaluated using finite element method. Four cell lines are separated in this device containing red blood cells, platelets, white blood cells, and CTCs. Primarily, red blood cells and... 

    Wolfram-like syndrome with bicuspid aortic valve due to a homozygous missense variant in CDK13

    , Article Journal of Human Genetics ; Volume 66, Issue 10 , 2021 , Pages 1009-1018 ; 14345161 (ISSN) Acharya, A ; Raza, S. I ; Zeeshan Anwar, M ; Bharadwaj, T ; Liaqat, K ; Khokhar, M. A. S ; Everard, J. L ; Nasir, A ; Nickerson, D. A ; Bamshad, M. J ; Ansar, M ; Schrauwen, I ; Ahmad, W ; Leal, S. M ; Sharif University of Technology
    Springer Nature  2021
    Abstract
    Background: Wolfram syndrome (WFS) is characterized by deafness, diabetes mellitus, and diabetes insipidus along with optic atrophy. WFS has an autosomal recessive mode of inheritance and is due to variants in WFS1 and CISD2. Methods: We evaluated the underlying molecular etiology of three affected members of a consanguineous family with hearing impairment, bicuspid aortic valve, diabetes mellitus and insipidus, clinodactyly, and gastrointestinal tract abnormalities via exome sequencing approach. We correlated clinical and imaging data with the genetic findings and their associated phenotypes. Results: We identified a homozygous missense variant p.(Asn1097Lys) in CDK13, a gene previously... 

    Development of a genetic algorithm based biomechanical simulation of sagittal lifting tasks

    , Article Biomedical Engineering - Applications, Basis and Communications ; Volume 17, Issue 1 , 2005 , Pages 12-18 ; 10162372 (ISSN) Gündoǧdu, Ö ; Anderson, K. S ; Parnianpour, M ; Sharif University of Technology
    Institute of Biomedical Engineering  2005
    Abstract
    Fibrin sealant and platelet gels are human blood-derived, biodegradable, non toxic, surgical products obtained by mixing a fibrinogen concentrate or a platelet rich plasma with thrombin, respectively. Fibrin sealant is now a well known surgical tool increasingly used to stop or control bleeding, or to provide air and fluid tightness in many surgical situations. Platelet gels are newly developed preparations that are of specific interest because they contain numerous physiological growth factors and cytikines that are released upon the activation of blood platelets by thrombin. These growth factors, including PDGF, TGF-β1, BMP, and VEGF have been shown to stimulate cell growth and... 

    Numerical analysis of a dielectrophoresis field-flow fractionation device for the separation of multiple cell types

    , Article Journal of Separation Science ; Volume 40, Issue 20 , 2017 , Pages 4067-4075 ; 16159306 (ISSN) Shamloo, A ; Kamali, A ; Sharif University of Technology
    Abstract
    In this study, a dielectrophoresis field-flow fractionation device was analyzed using a numerical simulation method and the behaviors of a set of different cells were investigated. By reducing the alternating current frequency of the electrodes from the value used in the original setup configuration and increasing the number of exit channels, total discrimination in cell trajectories and subsequent separation of four cell types were achieved. Cells were differentiated based on their size and dielectric response that are represented in their real part of Clausius–Mossotti factor at different frequencies. A number of novel designs were also proposed based on the original setup configuration.... 

    Developing hyaluronic acid microgels for sustained delivery of platelet lysate for tissue engineering applications

    , Article International Journal of Biological Macromolecules ; Volume 144 , 2020 , Pages 837-846 Jooybar, E ; Abdekhodaie, M. J ; Karperien, M ; Mousavi, A ; Alvi, M ; Dijkstra, P. J ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    Platelet lysate (PL), a blood product that contains high concentrations of growth factors (GFs), can be considered as a cost-effective source of multiple GFs. In this study, hyaluronic acid (HA) based microgels were developed for delivery of PL proteins. Spherical microgel were prepared using a water in oil emulsion method. First, hyaluronic acid was grafted with tyramine groups, after which prepared microdroplets were crosslinked via an enzymatic reaction in the presence of hydrogen peroxide and horseradish peroxidase. Because of electrostatic interactions, these microgels are promising carriers for positively charged proteins entrapment like most of the GFs. When microgels are incubated in... 

    A regenerative approach towards recovering the mechanical properties of degenerated intervertebral discs: Genipin and platelet-rich plasma therapies

    , Article Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine ; Volume 231, Issue 2 , 2017 , Pages 127-137 ; 09544119 (ISSN) Nikkhoo, M ; Wang, J. L ; Abdollahi, M ; Hsu, Y. C ; Parnianpour, M ; Khalaf, K ; Sharif University of Technology
    SAGE Publications Ltd  2017
    Abstract
    Degenerative disc disease, associated with discrete structural changes in the peripheral annulus and vertebral endplate, is one of the most common pathological triggers of acute and chronic low back pain, significantly depreciating an individual's quality of life and instigating huge socioeconomic costs. Novel emerging therapeutic techniques are hence of great interest to both research and clinical communities alike. Exogenous crosslinking, such as Genipin, and platelet-rich plasma therapies have been recently demonstrated encouraging results for the repair and regeneration of degenerated discs, but there remains a knowledge gap regarding the quantitative degree of effectiveness and... 

    Nanomedicine and advanced technologies for burns: Preventing infection and facilitating wound healing

    , Article Advanced Drug Delivery Reviews ; Volume 123 , 2018 , Pages 33-64 ; 0169409X (ISSN) Mofazzal Jahromi, M. A ; Sahandi Zangabad, P ; Moosavi Basri, S. M ; Sahandi Zangabad, K ; Ghamarypour, A ; Aref, A. R ; Karimi, M ; Hamblin, M. R ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    According to the latest report from the World Health Organization, an estimated 265,000 deaths still occur every year as a direct result of burn injuries. A widespread range of these deaths induced by burn wound happens in low- and middle-income countries, where survivors face a lifetime of morbidity. Most of the deaths occur due to infections when a high percentage of the external regions of the body area is affected. Microbial nutrient availability, skin barrier disruption, and vascular supply destruction in burn injuries as well as systemic immunosuppression are important parameters that cause burns to be susceptible to infections. Topical antimicrobials and dressings are generally... 

    Recent advances in the design and applications of amyloid-β peptide aggregation inhibitors for Alzheimer’s disease therapy

    , Article Biophysical Reviews ; Volume 11, Issue 6 , 2019 , Pages 901-925 ; 18672450 (ISSN) Jokar, S ; Khazaei, S ; Behnammanesh, H ; Shamloo, A ; Erfani, M ; Beiki, D ; Bavi, O ; Sharif University of Technology
    Springer  2019
    Abstract
    Alzheimer’s disease (AD) is an irreversible neurological disorder that progresses gradually and can cause severe cognitive and behavioral impairments. This disease is currently considered a social and economic incurable issue due to its complicated and multifactorial characteristics. Despite decades of extensive research, we still lack definitive AD diagnostic and effective therapeutic tools. Consequently, one of the most challenging subjects in modern medicine is the need for the development of new strategies for the treatment of AD. A large body of evidence indicates that amyloid-β (Aβ) peptide fibrillation plays a key role in the onset and progression of AD. Recent studies have reported...