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tissue-culture-polystyrenes
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Influence of Fe3O4 nanoparticles in hydroxyapatite scaffolds on proliferation of primary human fibroblast cells
, Article Journal of Materials Engineering and Performance ; 2016 , Pages 1-9 ; 10599495 (ISSN) ; Aghaie, E ; Nadernezhad, A ; Zargarzadeh, M ; Khakzad, A ; Shakeri, M. S ; Beygi Khosrowshahi, Y ; Siadati, M. H ; Sharif University of Technology
Springer New York LLC
2016
Abstract
Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of...
The synergistic effect of surface topography and sustained release of TGF-β1 on myogenic differentiation of human mesenchymal stem cells
, Article Journal of Biomedical Materials Research - Part A ; Volume 104, Issue 7 , 2016 , Pages 1610-1621 ; 15493296 (ISSN) ; Mashayekhan, S ; Vakilian, S ; Ardeshirylajimi, A ; Soleimani, M ; Sharif University of Technology
John Wiley and Sons Inc
Abstract
A combination of topographical cues and controlled release of biochemical factors is a potential platform in controlling stem cells differentiation. In this study the synergistic effect of nanotopography and sustained release of biofunctional transforming growth factor beta 1 (TGF-β1) on differentiation of human Wharton's Jelly-derived mesenchymal stem cell (hWJ-derived UC-MSCs) toward myogenic lineage was investigated. In order to achieve a sustained release of TGF-β1, this factor was encapsulated within chitosan nanoparticles. Afterwards the aligned composite mats were fabricated using poly-E-caprolacton (PCL) containing TGF-β1-loaded chitosan nanoparticles and poly-L-lactic acid (PLLA)....
Physical, morphological, and biological studies on PLA/nHA composite nanofibrous webs containing equisetum arvense herbal extract for bone tissue engineering
, Article Journal of Applied Polymer Science ; Volume 134, Issue 39 , 2017 ; 00218995 (ISSN) ; Jafari, S. H ; Zahedi, P ; Bagheri, R ; Hajiaghaee, R ; Sharif University of Technology
Abstract
A series of herbal extract incorporated into poly(lactic acid) (PLA) composite nanofibrous scaffolds were successfully prepared by using electrospinning technique. Equisetum arvense extract (EE) and nanohydroxyapatite (nHA) in different quantities were loaded into PLA solution to fabricate composite nanofibrous webs under various electrospinning conditions. Uniform nanofibers were obtained with an average diameter of 157 ± 47 nm in the case of those containing the herbal extract. Characterization of the webs was carried out by means of Fourier transform infrared (FTIR) spectroscopy, field emission-scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and...