Search for: tissue-engineering
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    Skin diseases modeling using combined tissue engineering and microfluidic technologies

    , Article Advanced Healthcare Materials ; Volume 5, Issue 19 , 2016 , Pages 2459-2480 ; 21922640 (ISSN) Mohammadi, M. H ; Heidary Araghi, B ; Beydaghi, V ; Geraili, A ; Moradi, F ; Jafari, P ; Janmaleki, M ; Valente, K. P ; Akbari, M ; Sanati Nezhad, A ; Sharif University of Technology
    Wiley-VCH Verlag 
    In recent years, both tissue engineering and microfluidics have significantly contributed in engineering of in vitro skin substitutes to test the penetration of chemicals or to replace damaged skins. Organ-on-chip platforms have been recently inspired by the integration of microfluidics and biomaterials in order to develop physiologically relevant disease models. However, the application of organ-on-chip on the development of skin disease models is still limited and needs to be further developed. The impact of tissue engineering, biomaterials and microfluidic platforms on the development of skin grafts and biomimetic in vitro skin models is reviewed. The integration of tissue engineering and... 

    Fabrication and evaluation of chitosan/gelatin/PVA hydrogel incorporating honey for wound healing applications: An in vitro, in vivo study

    , Article International Journal of Pharmaceutics ; 2020 Shamloo, A ; Aghababaie, Z ; Afjoul, H ; Jami, M ; Bidgoli, M. R ; Vossoughi, M ; Ramazani, A ; Kamyabhesari, K ; Sharif University of Technology
    Elsevier B.V  2020
    In this study, physically cross-linked hydrogels were developed by freezing-thawing method while different concentrations of honey were included into the hydrogels for accelerated wound healing. The hydrogel was composed of chitosan, polyvinyl alcohol (PVA), and gelatin with the ratio of 2:1:1 (v/v), respectively. Further, the effect of honey concentrations on antibacterial properties, and cell behavior was investigated. In vivo studies, including wound healing mechanism using rat model and histological analysis of section tissue samples were performed. The results illustrated that the incorporation of honey in hydrogels increased the ultimate strain of hydrogels approximately two times,... 

    Fabrication and characterization of biaxially electrospun collagen/alginate nanofibers, improved with Rhodotorula mucilaginosa sp. GUMS16 produced exopolysaccharides for wound healing applications

    , Article International Journal of Biological Macromolecules ; 2021 ; 01418130 (ISSN) Ashraf, S ; Parivar, K ; Hayati Roodbari, N ; Mashayekhan, S ; Amini, N ; Sharif University of Technology
    Elsevier B.V  2021
    Fabrication of scaffolds with enhanced mechanical properties and desirable cellular compatibility is critical for numerous tissue engineering applications. This study was aimed at fabrication and characterization of a nanofiber skin substitute composed of collagen (Col)/sodium alginate (SA)/ polyethylene oxide (PEO)/Rhodotorula mucilaginosa sp. GUMS16 produced exopolysaccharides (EPS) were prepared using the biaxial electrospinning technique. This study used collagen extracted from the bovine tendon as a natural scaffold, sodium alginate as an absorber of excess wound fluids, and GUMS16 produced exopolysaccharides as an antioxidant. Collagen was characterized using FTIR and EDS analyses. The... 

    Fabrication and characterization of electrospun poly-L-lactide/gelatin graded tubular scaffolds: Toward a new design for performance enhancement in vascular tissue engineering

    , Article Progress in Natural Science: Materials International ; Volume 25, Issue 5 , October , 2015 , Pages 405–413 ; 10020071 (ISSN) Yazdanpanah, A ; Tahmasbi, M ; Amoabediny, G ; Nourmohammadi, J ; Moztarzadeh, F ; Mozafari, M ; Sharif University of Technology
    Elsevier  2015
    In this study, a new design of graded tubular scaffolds have been developed for the performance enhancement in vascular tissue engineering. The graded poly-L-lactide (PLLA) and gelatin fibrous scaffolds produced by electrospining were then characterized. The morphology, degradability, porosity, pore size and mechanical properties of four tubular scaffolds (graded PLLA/gelatin, layered PLLA/gelatin, PLLA and gelatin scaffolds) have been investigated. The tensile tests demonstrated that the mechanical strength and also the estimated burst pressure of the graded scaffolds were significantly increased in comparison with the layered and gelatin scaffolds. This new design, resulting in an increase... 

    Geometry optimization of a fibrous scaffold based on mathematical modelling and CFD simulation of a dynamic cell culture

    , Article Computer Aided Chemical Engineering ; Volume 38 , 2016 , Pages 1413-1418 ; 15707946 (ISSN); 9780444634283 (ISBN) Tajsoleiman, T ; Abdekhodaie, M. J ; Gernaey, K. V ; Krühne, U ; Kravanja, Z ; Bogataj, M ; Sharif University of Technology
    Elsevier B.V  2016
    In tissue engineering, the development of a tissue essentially depends on supply of an adequate amount of nutrients and the design of a proper biophysical micro-environment for cells. The limitation of the available initial number of cells, expensive substances and time consuming experiments are the main bottlenecks in this type of processes. In this regard, mathematical modelling and computational fluid dynamics simulation (CFD) are powerful tools to identify an efficient and optimized design by providing reliable insights of the process. This study presents a mathematical model and CFD simulation of cartilage cell culture under a perfusion flow, which allows not only to characterize the... 

    Piezoelectric conductive electrospun nanocomposite PCL/Polyaniline/Barium Titanate scaffold for tissue engineering applications

    , Article Scientific Reports ; Volume 12, Issue 1 , 2022 ; 20452322 (ISSN) Peidavosi, N ; Azami, M ; Beheshtizadeh, N ; Ramazani Saadatabadi, A ; Sharif University of Technology
    Nature Research  2022
    Recent trends in tissue engineering technology have switched to electrical potentials generated through bioactive scaffolds regarding their appropriate effects on cell behaviors. Preparing a piezo-electrical stimuli scaffold with high electrical conductivity for bone and cartilage tissue regeneration is the ultimate goal of the present study. Here, Barium Titanate nanoparticles (BaTiO3 NPs) were used as piezoelectric material and highly conductive binary doped Polyaniline nanoparticles (PANI NPs) were synthesized by oxidative polymerization. Polycaprolactone (PCL) was applied as carrier substrate polymer and conductive spun nanofibrous scaffolds of PCL/PANI composites were prepared in two... 

    Prevascularized micro-/nano-sized spheroid/bead aggregates for vascular tissue engineering

    , Article Nano-Micro Letters ; Volume 13, Issue 1 , 2021 ; 23116706 (ISSN) Rahimnejad, M ; Nasrollahi Boroujeni, N ; Jahangiri, S ; Rabiee, N ; Rabiee, M ; Makvandi, P ; Akhavan, O ; Varma, R. S ; Sharif University of Technology
    Springer Science and Business Media B.V  2021
    Efficient strategies to promote microvascularization in vascular tissue engineering, a central priority in regenerative medicine, are still scarce; nano- and micro-sized aggregates and spheres or beads harboring primitive microvascular beds are promising methods in vascular tissue engineering. Capillaries are the smallest type and in numerous blood vessels, which are distributed densely in cardiovascular system. To mimic this microvascular network, specific cell components and proangiogenic factors are required. Herein, advanced biofabrication methods in microvascular engineering, including extrusion-based and droplet-based bioprinting, Kenzan, and biogripper approaches, are deliberated with... 

    An optimized procedure to develop a 3-dimensional microfluidic hydrogel with parallel transport networks

    , Article International Journal for Numerical Methods in Biomedical Engineering ; 2018 ; 20407939 (ISSN) Jafarkhani, M ; Salehi, Z ; Shokrgozar, M. A ; Mashayekhan, S ; Sharif University of Technology
    Wiley-Blackwell  2018
    The development of microfluidic hydrogels is an attractive method to generate continuous perfusion, induce vascularization, increase solute delivery, and ultimately improve cell viability. However, the transport processes in many in vitro studies still have not been realized completely. To address this problem, we have developed a microchanneled hydrogel with different collagen type I concentrations of 1, 2, and 3 wt% and assessed its physical properties and obtained diffusion coefficient of nutrient within the hydrogel. It is well known that microchannel geometry has critical role in maintaining stable perfusion rate. Therefore, in this study, a computational modeling was applied to... 

    Design and Fabrication of Nerve Guidance Conduit for Perioheral Nerve Regeneration based on Gelatin/Graphene

    , M.Sc. Thesis Sharif University of Technology Mohammadi, Mohammad (Author) ; Ramazani Saadatabadi, Ahmad (Supervisor) ; Mashayekhan, Shohreh (Co-Advisor)
    The the nervous system as a most comlicated body system , plays an important and vital role for the body systems. damage to the peripheral nervous system result in nervous system disorders which claissified to Neuropraxia, Axonotmesis and Neurotmesis based on damage itensity. tissue engineering considered as one of the repairing nervous damage way, which by creating 3D substrat (scaffold) with proper physical structure, increses possibility of adhesion, growth and proliferation of cells to increase regeneration rate of damaged nerve. in this study, a combination of dual-electrospinning and rolling the spun film used to preparate a nerve guidance conduit (NGC) based on gelatin, PCL and... 

    Smart polymeric hydrogels for cartilage tissue engineering: A review on the chemistry and biological functions

    , Article Biomacromolecules ; Volume 17, Issue 11 , 2016 , Pages 3441-3463 ; 15257797 (ISSN) Eslahi, N ; Abdorahim, M ; Simchi, A ; Sharif University of Technology
    American Chemical Society 
    Stimuli responsive hydrogels (SRHs) are attractive bioscaffolds for tissue engineering. The structural similarity of SRHs to the extracellular matrix (ECM) of many tissues offers great advantages for a minimally invasive tissue repair. Among various potential applications of SRHs, cartilage regeneration has attracted significant attention. The repair of cartilage damage is challenging in orthopedics owing to its low repair capacity. Recent advances include development of injectable hydrogels to minimize invasive surgery with nanostructured features and rapid stimuli-responsive characteristics. Nanostructured SRHs with more structural similarity to natural ECM up-regulate cell-material... 

    Microcarriers Development for Stem Cells Cultivation

    , M.Sc. Thesis Sharif University of Technology Karimian Shamsabadi, Mohammad (Author) ; Mashayekhan, Shohre (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    In general, repair and regeneration of damaged tissues or lost organ is base definition of a technology named tissue engineering. Tissue engineering technology provides innovative path to achieve better medical treatment, including different methods which manipulate natural or synthetic biomaterials mimicking stem cells environmental condition inside the body, in order to facilitate desired cell expansion and differentiation. Recently, application of cell carriers in form of microcarriers which are injectable, biodegradable and biocompatible have attracted significant attention. Due to increasing effectiveness in animal cell culture.
    The aim of this work was fabrication of... 

    Fabrication of Artificial skin with Electrospun of Nanofiber made of PCL and PVA

    , M.Sc. Thesis Sharif University of Technology Mohseni, Mina (Author) ; Shamloo, Amir (Supervisor) ; Naghdabadi, Reza (Co-Advisor) ; Vosooghi, Manoochehr (Co-Advisor) ; Firoozbakhsh, Keikhosro (Co-Advisor)
    The main goal of this project is to fabricate the scaffolds including Polycaprolactone and Polyvinyle alcohol nanofibers in order to use as artificial skin. For this purpose, electrospinning parameters have been optimized for various injection rates and the scaffolds containing different percent of PCL and PVA have been made. By investigating the cell growth on these scaffolds, the one made up PVA/PCL (30% ) has been introduced as the best scaffold. To improve biological properties of chosen scaffold, a layer of collagen was coated. The angiogenesis capacity has been improved by adding Heparin to PVA nanofibers. Based on assay of heparin release, it was shown that Heparin is released within... 

    Investigating the Optimum Conditions for Cell Growth and Behavior on Hydrogel Surfaces

    , M.Sc. Thesis Sharif University of Technology Hajiabbas, Maryam (Author) ; Mashayekhan, Shohre (Supervisor) ; Maghsudi, Vida (Supervisor)
    Generally, the concept of producing ‘spare parts’ of the body for replacement of damaged or lost organs lies at the core of the varied biotechnological practices referred as tissue engineering. Tissue engineering is an interdisciplinary field that incorporates principles of engineering with the life sciences. Tissue engineering is based on three principle; cells, scaffolds for cells expansion, attachment as an environment like ECM and growth factors. These things together can help tissue engineers to provide microenvironments which are suitable for special cells. The most important thing in this kind of works is the ability to simulate environment for cells the same as body. According to the... 

    Chitosan/Gelatin/Mg Substituted Hydroxyapatite Nano Composite Scaffold for Bone Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Azamian, Fariba (Author) ; Mohammadi, Mohammad Reza (Supervisor) ; Fathi, Mohammad Hossein (Co-Advisor)
    The present study is work on fabrication and characterization of a chitosan/ gelatin/ Mg substituted hydroxyapatite nano composite scaffold for bone tissue engineering. At the first step pure and Mg-substituted hydroxyapatite (HA) (Ca10-xMgx(PO4) 6OH2) nano-hexagonal rods with 14–45 nmdiameter.for this, calcium nitrate, magnesium phosphate hydrate and potassium dihydrogenphosphate were used as precursors for Ca, Mg, and P, respectively. Calculated amounts of magnesium ions (Mg+2) especially from 0 to 8% (molar ratio) were incorporated as substituted into the calcium sol solution. Deionized water was used as a diluting media for HA sol preparation and ammonia was used to adjust the pH= 9.... 

    Efficient computational design of a scaffold for cartilage cell regeneration

    , Article Bioengineering ; Volume 5, Issue 2 , 24 April , 2018 ; 23065354 (ISSN) Tajsoleiman, T ; Abdekhodaie, M. J ; Gernaey, K. V ; Kruhne, U ; Sharif University of Technology
    MDPI AG  2018
    Due to the sensitivity of mammalian cell cultures, understanding the influence of operating conditions during a tissue generation procedure is crucial. In this regard, a detailed study of scaffold based cell culture under a perfusion flow is presented with the aid of mathematical modelling and computational fluid dynamics (CFD). With respect to the complexity of the case study, this work focuses solely on the effect of nutrient and metabolite concentrations, and the possible influence of fluid-induced shear stress on a targeted cell (cartilage) culture. The simulation set up gives the possibility of predicting the cell culture behavior under various operating conditions and scaffold designs.... 

    Polysaccharide-based nanocomposites for biomedical applications: a critical review

    , Article Nanoscale Horizons ; Volume 7, Issue 10 , 2022 , Pages 1136-1160 ; 20556756 (ISSN) Shokrani, H ; Shokrani, A ; Sajadi, S. M ; Khodadadi Yazdi, M ; Seidi, F ; Jouyandeh, M ; Zarrintaj, P ; Kar, S ; Kim, S. J ; Kuang, T ; Rabiee, N ; Hejna, A ; Saeb, M. R ; Ramakrishna, S ; Sharif University of Technology
    Royal Society of Chemistry  2022
    Polysaccharides (PSA) have taken specific position among biomaterials for advanced applications in medicine. Nevertheless, poor mechanical properties are known as the main drawback of PSA, which highlights the need for PSA modification. Nanocomposites PSA (NPSA) are a class of biomaterials widely used as biomedical platforms, but despite their importance and worldwide use, they have not been reviewed. Herein, we critically reviewed the application of NPSA by categorizing them into generic and advanced application realms. First, the application of NPSA as drug and gene delivery systems, along with their role in the field as an antibacterial platform and hemostasis agent is discussed. Then,... 

    Electrically conductive carbon-based (bio)-nanomaterials for cardiac tissue engineering

    , Article Bioengineering and Translational Medicine ; 2022 ; 23806761 (ISSN) Jalilinejad, N ; Rabiee, M ; Baheiraei, N ; Ghahremanzadeh, R ; Salarian, R ; Rabiee, N ; Akhavan, O ; Zarrintaj, P ; Hejna, A ; Saeb, M. R ; Zarrabi, A ; Sharifi, E ; Yousefiasl, S ; Zare, E. N ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    A proper self-regenerating capability is lacking in human cardiac tissue which along with the alarming rate of deaths associated with cardiovascular disorders makes tissue engineering critical. Novel approaches are now being investigated in order to speedily overcome the challenges in this path. Tissue engineering has been revolutionized by the advent of nanomaterials, and later by the application of carbon-based nanomaterials because of their exceptional variable functionality, conductivity, and mechanical properties. Electrically conductive biomaterials used as cell bearers provide the tissue with an appropriate microenvironment for the specific seeded cells as substrates for the sake of... 

    Emerging bioengineering strategies for regulating stem cell fate: Scaffold physical and biochemical cues

    , Article Tissue Engineering: Current Status and Challenges ; 2022 , Pages 125-156 ; 9780128240649 (ISBN) Sharareh Mahdavi, S ; Mashayekhan, S ; Sharif University of Technology
    Elsevier  2022
    Stem cell therapy has been introduced as an emerging approach for injured tissue regeneration. This chapter addresses developing regenerative medicine techniques for controlling stem cell behavior. Recent studies have been reviewed and novel approaches have been divided into four main categories: 3D bioprinting, lithography, microfluidics, and electrospinning. Moreover, the impact of applied biophysical and/or biochemical cues to the designed scaffold on controlling stem cell activity has been discussed. The potential of using stem cells for various soft and hard tissue regenerations has been explored in different bioengineered scaffolds and the applied techniques for controlling stem cell... 

    Investigation on Conductivity and Piezoelectric Effects on Biological Properties of Conductive Nerve Regenerating Scaffolds by Nano Composite of Gelatin/Polyvinyl Fluoride/Graphene

    , M.Sc. Thesis Sharif University of Technology Sahrayi, Hamid Reza (Author) ; Ramazani Saadat Abadi, Ahmad (Supervisor) ; Bakhshandeh Abkenar, Haleh (Supervisor) ; Atyabi, Mohammad (Co-Supervisor)
    Neural system in human body due to limitated cell proliferation requires high care, because the damage causes serious hurts and disables different tissues. Limit regeneration in central neural system can cause different neural diseases including brain exhaustions specially in old people, but the peripheral neural system may have been disabled and intrupt some other tissue beacuase of possible external injuries before aging. So that while some damages like neural fibers severing, some new medical care methods are required. Some of these methods are outograft, allograft and xenograft which implants neural tissues either between other patient's tissues or from somebody else's; however these... 

    Preparation and Characterization of Chitosan-Gelatin/Polyanilne-Graphene-CNT Nanocomposite for Neural Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Aein Jamshid, Mohammad (Author) ; Ramazani Saadatabadi, Ahmad (Supervisor)
    Repairing and regeneration of neural tissues have attracted considerable attention due to its direct effect on the quality of patients’ lives. Recent developments in nanotechnology and tissue engineering have facilitated their comprehensive applications in the treatment of neurological diseases, and several effective ways have been proposed to repair defects in neural tissues. The main objective of the researches in this field is to regulate cellular behavior and tissue progression through the design and development of synthetic extracellular matrices, such as novel biomaterials, that enhance cell culture and tissue regeneration. Natural polymers, such as chitosan and gelatin, are suitable...