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    Modifying Collagen Membrane Used for Guided Tissue Regeneration

    , M.Sc. Thesis Sharif University of Technology Ansarizadeh, Mohammad Hassan (Author) ; Mashayekhan, Shohreh (Supervisor) ; Saadatmand, Maryam (Supervisor) ; Khoshzaban, Ahad (Co-Advisor)
    In this study, lyophilized advance platelet rich fibrin (A-PRF) was used in combination with collagen membrane for the first time to accelerate bone regeneration as a result of growth factor release. Firstly, collagen was extracted from calf skin and analyzed using SDS-PAGE. FTIR has shown that EDC/NHS were chemically crosslinked collagen to collagen and collagen to chitosan. The morphology of collagen, collagen/chitosan composite membrane with different chitosan content, lyophilized A-PRF attached to membrane were showed using SEM images. The pore sizes were varied from 100 to 300 µm. Response surface methodology (RSM) was used to design experimental condition and to correlate the effect... 

    Development of a Polymeric Scaffold for Periodontal Regeneration

    , M.Sc. Thesis Sharif University of Technology Rahimi Nasrabadi, Kosar (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor)
    Periodontitis is a common inflammatory disease that affects the periodontium.Periodontium includes two hard tissues of cementum and alveolar bone and also soft tissue of periodontal ligament. The appropriate function is based on the consistency and accurate interaction of them. The complex structure, the low potential of the body for spontaneous healing, and technical problems such as bacteria accumulation, limited access, and small operating field cause no complete treatment can be achieved until now.In this project, at first collagen type I was extracted from Bovine Achilles tendon. Then, polymer modification was done to 39.95 µg/mg (Tyramine/ Collagen). An In situ gel based on modified... 

    Microfluidic devices as invitro microenvironments for -stem cell culture

    , Article Proceedings of the IASTED International Conference on Biomedical Engineering, BioMed 2014 ; 2014 , pp. 83-88 Shamloo, A ; Abeddoust, M ; Mehboudi, N ; Sharif University of Technology
    Many potential therapies are currently being studied that may promote neural regeneration and guide regenerating axons to form correct connections following injury. It has been shown that adult neurons have some limited regenerative capabilities, and the lack of connection formation between neurons is not an intrinsic inability of these cells to form axons after being damaged, but rather the inhibitory microenvironment of the injured tissue prevents regeneration. In this study, the polarization and chemotaxis of neuronal stem cells (NSC) in response to quantified gradients of nerve growth factor (NGF) was examined. To accomplish this, a microfluidic device was designed and fabricated to... 

    Rolled graphene oxide foams as three-dimensional scaffolds for growth of neural fibers using electrical stimulation of stem cells

    , Article Carbon ; Volume 97 , 2016 , Pages 71-77 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Shirazian, S. A ; Rahighi, R ; Sharif University of Technology
    Elsevier Ltd 
    Graphene oxide foam (GOF) layers with thicknesses of ∼15-50 μm and density of ∼10 graphene oxide (GO) sheets/μm were fabricated by precipitation of chemically exfoliated GO sheets in an aqueous suspension at ∼80 °C under UV irradiation. Then, rolled GOFs with desirable scales were developed as electrically conductive 3D-scaffolds and applied in directional growth of neural fibers, through differentiation of human neural stem cells (hNSCs) into neurons under an electrical stimulation. X-ray photoelectron spectroscopy indicated that the UV irradiation resulted in partial deoxygenation of the layers. Scanning electron microscopy and Raman spectroscopy confirmed the presence of multilayer GO... 

    Three-dimensional bioprinting of functional skeletal muscle tissue using gelatin methacryloyl-alginate bioinks

    , Article Micromachines ; Volume 10, Issue 10 , 2019 ; 2072666X (ISSN) Seyedmahmoud, R ; Çelebi Saltik, B ; Barros, N ; Nasiri, R ; Banton, E ; Shamloo, A ; Ashammakhi, N ; Dokmeci, M. R ; Ahadian, S ; Sharif University of Technology
    MDPI AG  2019
    Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL... 

    The impact of zirconium oxide nanoparticles content on alginate dialdehyde-gelatin scaffolds in cartilage tissue engineering

    , Article Journal of Molecular Liquids ; Volume 335 , 2021 ; 01677322 (ISSN) Ghanbari, M ; Salavati Niasari, M ; Mohandes, F ; Firouzi, Z ; Mousavi, S.-D ; Sharif University of Technology
    Elsevier B.V  2021
    The desire to regenerate and repair native tissues can be immediately performed by multiple tissue engineering procedures. Gelatin and alginate are biocompatible and biodegradable polymers. The addition of ZrO2 nanoparticles (NPs) into the alginate-gelatin hydrogel is considered to improve mechanical and chemical properties. Therefore, nanocomposite hydrogels have been manufactured by the freeze-drying procedure utilizing oxidized alginate-gelatin with ZrO2 NPs as a reinforcement. The fabricated nanocomposite hydrogels were character-ized by FTIR, FESEM, and rheometer. The hydrogels containing a higher ZrO2 NPs content (1.5%) have better mechanical properties than the hydrogels without NPs.... 

    Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane

    , Article International Journal of Biological Macromolecules ; Volume 125 , 2019 , Pages 383-391 ; 01418130 (ISSN) Ansarizadeh, M ; Mashayekhan, S ; Saadatmand, M ; Sharif University of Technology
    Elsevier B.V  2019
    In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa. Cell viability of MSCs was improved by both... 

    Fabrication of a highly ordered hierarchically designed porous nanocomposite via indirect 3D printing: Mechanical properties and in vitro cell responses

    , Article Materials and Design ; Volume 88 , 2015 , Pages 924-931 ; 02641275 (ISSN) Tamjid, E ; Simchi, A ; Sharif University of Technology
    Elsevier Ltd  2015
    Design and development of biodegradable scaffolds with highly uniform and controlled internal structure that stimulate tissue regeneration are the focus of many studies. The aim of this work is to apply a modified three-dimensional (3D) printing process to fabricate polymer-matrix composites with controlled internal architecture. Computationally-designed plaster molds with various pore sizes in the range of 300-800. μm were prepared by employing 3D printing of a water-based binder. The molds were converted to ε-polycaprolactone (PCL) and PCL/bioactive glass (BG) composite scaffolds by solvent casting and freeze drying methods. Optical and electron microscopy studies revealed that the pore... 

    Influence of Fe3O4 nanoparticles in hydroxyapatite scaffolds on proliferation of primary human fibroblast cells

    , Article Journal of Materials Engineering and Performance ; 2016 , Pages 1-9 ; 10599495 (ISSN) Maleki Ghaleh, H ; Aghaie, E ; Nadernezhad, A ; Zargarzadeh, M ; Khakzad, A ; Shakeri, M. S ; Beygi Khosrowshahi, Y ; Siadati, M. H ; Sharif University of Technology
    Springer New York LLC  2016
    Modern techniques for expanding stem cells play a substantial role in tissue engineering: the raw material that facilitates regeneration of damaged tissues and treats diseases. The environmental conditions and bioprocessing methods are the primary determinants of the rate of cultured stem cell proliferation. Bioceramic scaffolds made of calcium phosphate are effective substrates for optimal cell proliferation. The present study investigates the effects of two bioceramic scaffolds on proliferating cells in culture media. One scaffold was made of hydroxyapatite and the other was a mixture of hydroxyapatite and ferromagnetic material (Fe3O4 nanoparticles). Disk-shaped (10 mm × 2 mm) samples of... 

    Drug delivery systems and materials for wound healing applications

    , Article Advanced Drug Delivery Reviews ; Volume 127 , 2018 , Pages 138-166 ; 0169409X (ISSN) Saghazadeh, S ; Rinoldi, C ; Schot, M ; Saheb Kashaf, S ; Sharifi, F ; Jalilian, E ; Nuutila, K ; Giatsidis, G ; Mostafalu, P ; Derakhshandeh, H ; Yue, K ; Swieszkowski, W ; Memic, A ; Tamayol, A ; Khademhosseini, A ; Sharif University of Technology
    Elsevier B.V  2018
    Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug... 

    A hydrogen-bonded extracellular matrix-mimicking bactericidal hydrogel with radical scavenging and hemostatic function for ph-responsive wound healing acceleration

    , Article Advanced Healthcare Materials ; 2020 Ahmadian, Z ; Correia, A ; Hasany, M ; Figueiredo, P ; Dobakhti, F ; Eskandari, M. R ; Hosseini, H ; Abiri, R ; Khorshid, S ; Hirvonen, J ; Santos, H. A ; Shahbazi, M. A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Generation of reactive oxygen species, delayed blood clotting, prolonged inflammation, bacterial infection, and slow cell proliferation are the main challenges of effective wound repair. Herein, a multifunctional extracellular matrix-mimicking hydrogel is fabricated through abundant hydrogen bonding among the functional groups of gelatin and tannic acid (TA) as a green chemistry approach. The hydrogel shows adjustable physicochemical properties by altering the concentration of TA and it represents high safety features both in vitro and in vivo on fibroblasts, red blood cells, and mice organs. In addition to the merit of facile encapsulation of cell proliferation-inducing hydrophilic drugs,... 

    Bioengineering approaches for corneal regenerative medicine

    , Article Tissue Engineering and Regenerative Medicine ; Volume 17, Issue 5 , July , 2020 , Pages 567-593 Mahdavi, S. S ; Abdekhodaie, M. J ; Mashayekhan, S ; Baradaran Rafii, A ; Djalilian, A. R ; Sharif University of Technology
    Korean Tissue Engineering and Regenerative Medicine Society  2020
    Background:: Since the cornea is responsible for transmitting and focusing light into the eye, injury or pathology affecting any layer of the cornea can cause a detrimental effect on visual acuity. Aging is also a reason for corneal degeneration. Depending on the level of the injury, conservative therapies and donor tissue transplantation are the most common treatments for corneal diseases. Not only is there a lack of donor tissue and risk of infection/rejection, but the inherent ability of corneal cells and layers to regenerate has led to research in regenerative approaches and treatments. Methods:: In this review, we first discussed the anatomy of the cornea and the required properties for... 

    Microfluidic technologies to engineer mesenchymal stem cell aggregates—applications and benefits

    , Article Biophysical Reviews ; Volume 12, Issue 1 , 2020 , Pages 123-133 Salehi, S. S ; Shamloo, A ; Kazemzadeh Hannani, S. K ; Sharif University of Technology
    Springer  2020
    Three-dimensional cell culture and the forming multicellular aggregates are superior over traditional monolayer approaches due to better mimicking of in vivo conditions and hence functions of a tissue. A considerable amount of attention has been devoted to devising efficient methods for the rapid formation of uniform-sized multicellular aggregates. Microfluidic technology describes a platform of techniques comprising microchannels to manipulate the small number of reagents with unique properties and capabilities suitable for biological studies. The focus of this review is to highlight recent studies of using microfluidics, especially droplet-based types for the formation, culture, and... 

    CaTiO3/α-TCP coatings on CP-Ti prepared via electrospinning and pulsed laser treatment for in-vitro bone tissue engineering

    , Article Surface and Coatings Technology ; Volume 401 , 2020 Yadi, M ; Esfahani, H ; Sheikhi, M ; Mohammadi, M ; Sharif University of Technology
    Elsevier B.V  2020
    In this study, the in-vitro bone regeneration ability of commercial pure titanium (CP-Ti) surface modified via electrospun polyvinylidene/hydroxyapatite (PVP/HA) masking and subsequent Nd-YAG pulsed laser treatment was investigated. The ratio of HA to PVP played a significant role in achieving a perfect homogenous mask on the CP-Ti. In the laser treatment process, the parameter of area scanning speed (ASS) had an important influence on the final surface morphology. A favorable range was defined for this parameter where these two conditions were satisfied: no PVP remaining and no severe substrate melting. Within a favorable range of ASS, as decreasing ASS exchanged the surface structure from... 

    Optimization of nano HA-SiC coating on AISI 316L medical grade stainless steel via electrophoretic deposition

    , Article Materials Letters ; Volume 285 , 2021 ; 0167577X (ISSN) Hosseini, M. R ; Ahangari, M ; Johar, M. H ; Allahkaram, S. R ; Sharif University of Technology
    Elsevier B.V  2021
    Biocompatible nanostructured coating plays an important role in enhancement of osseointegration ability of metallic implants. This study sets out to obtain optimized SiC concentration in Hydroxyapatite (HA) coating on AISI 316L stainless steel alloy through electrophoretic deposition method. Effect of SiC concentrations (1, 2, and 3%.wt) on the morphology, corrosion behaviour, and mechanical properties of the HA coating is investigated. Results show that SiC could obstruct the formation and growth of micro cracks in the HA coating where HA-3%SiC is considered as a crack free coating. Electrochemical tests reveal that SiC has improved the corrosion resistance of HA coating, and HA-3%SiC... 

    The effect of Ag incorporation on the characteristics of the polymer derived bioactive silicate phosphate glass-ceramic scaffolds

    , Article Boletin de la Sociedad Espanola de Ceramica y Vidrio ; 2021 ; 03663175 (ISSN) Paryab, A ; Godary, T ; Khalilifard, R ; Malek Khachatourian, A ; Abdollahi, F ; Abdollahi, S ; Sharif University of Technology
    Sociedad Espanola de Ceramica y Vidrio  2021
    In the bone tissue engineering field (BTE), it is of significant importance to develop bioactive multifunctional scaffolds with enhanced osteoconductivity, osteoinductivity, and antibacterial properties required for lost bone tissue regeneration. In this work, a bioactive glass-ceramic scaffold was manufactured via a novel polymer-derived ceramics (PDC) manufacturing method. To gain antibacterial properties, the silver ions were incorporated in controlled amount along with other precursors in the PDC processing stage. Microstructural and structural properties of the fabricated silicate-phosphate glass-ceramic scaffold were evaluated by scanning electron microscopy (SEM) equipped with energy... 

    Development of a genetic algorithm based biomechanical simulation of sagittal lifting tasks

    , Article Biomedical Engineering - Applications, Basis and Communications ; Volume 17, Issue 1 , 2005 , Pages 12-18 ; 10162372 (ISSN) Gündoǧdu, Ö ; Anderson, K. S ; Parnianpour, M ; Sharif University of Technology
    Institute of Biomedical Engineering  2005
    Fibrin sealant and platelet gels are human blood-derived, biodegradable, non toxic, surgical products obtained by mixing a fibrinogen concentrate or a platelet rich plasma with thrombin, respectively. Fibrin sealant is now a well known surgical tool increasingly used to stop or control bleeding, or to provide air and fluid tightness in many surgical situations. Platelet gels are newly developed preparations that are of specific interest because they contain numerous physiological growth factors and cytikines that are released upon the activation of blood platelets by thrombin. These growth factors, including PDGF, TGF-β1, BMP, and VEGF have been shown to stimulate cell growth and... 

    Design, preparation, and characterization of silk fibroin/carboxymethyl cellulose wound dressing for skin tissue regeneration applications

    , Article Polymer Engineering and Science ; Volume 62, Issue 9 , 2022 , Pages 2741-2749 ; 00323888 (ISSN) Farshi, P ; Salarian, R ; Rabiee, M ; Alizadeh, S ; Gholipourmalekabadi, M ; Ahmadi, S ; Rabiee, N ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    Silk fibroin (SF) has been broadly applied in wound dressing fabrication because of its proper features for wound healing. In this work, we developed a carboxymethyl cellulose (CMC)/gelatin blend film with different concentrations of glycerol, and modified the optimized film with an SF layer through electrospinning process. Tensile strength and cell viability evaluation of blend films demonstrated that the glycerol content of 3% could be suitable as the substrate layer for the two-layer wound dressing. The morphology of the blend film and electrospun nanofibers was obtained from scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). It concluded that... 

    Immunomodulating hydrogels as stealth platform for drug delivery applications

    , Article Pharmaceutics ; Volume 14, Issue 10 , 2022 ; 19994923 (ISSN) Rezaei, Z ; Yilmaz Aykut, D ; Tourk, F. M ; Bassous, N ; Barroso Zuppa, M ; Shawl, A. I ; Ashraf, S. S ; Avci, H ; Hassan, S ; Sharif University of Technology
    MDPI  2022
    Non-targeted persistent immune activation or suppression by different drug delivery platforms can cause adverse and chronic physiological effects including cancer and arthritis. Therefore, non-toxic materials that do not trigger an immunogenic response during delivery are crucial for safe and effective in vivo treatment. Hydrogels are excellent candidates that can be engineered to control immune responses by modulating biomolecule release/adsorption, improving regeneration of lymphoid tissues, and enhancing function during antigen presentation. This review discusses the aspects of hydrogel-based systems used as drug delivery platforms for various diseases. A detailed investigation on... 

    Magnetic nanocomposites for biomedical applications

    , Article Advances in Colloid and Interface Science ; Volume 308 , 2022 ; 00018686 (ISSN) Naghdi, M ; Ghovvati, M ; Rabiee, N ; Ahmadi, S ; Abbariki, N ; Sojdeh, S ; Ojaghi, A ; Bagherzadeh, M ; Akhavan, O ; Sharifi, E ; Rabiee, M ; Saeb, M. R ; Bolouri, K ; Webster, T. J ; Zare, E. N ; Zarrabi, A ; Sharif University of Technology
    Elsevier B.V  2022
    Tissue engineering and regenerative medicine have solved numerous problems related to the repair and regeneration of damaged organs and tissues arising from aging, illnesses, and injuries. Nanotechnology has further aided tissue regeneration science and has provided outstanding opportunities to help disease diagnosis as well as treat damaged tissues. Based on the most recent findings, magnetic nanostructures (MNSs), in particular, have emerged as promising materials for detecting, directing, and supporting tissue regeneration. There have been many reports concerning the role of these nano-building blocks in the regeneration of both soft and hard tissues, but the subject has not been...