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    Ethanol production from sugarcane bagasse by means of on-site produced and commercial enzymes; A comparative study

    , Article Periodica Polytechnica: Chemical Engineering ; Volume 56, Issue 2 , 2012 , Pages 91-96 ; 03245853 (ISSN) Shaibani, N ; Yaghmaei, S ; Andalibi, M. R ; Ghazvini, S ; Sharif University of Technology
    In this study ethanol was produced without using expensive commercial enzymes from sugarcane bagasse. Alkali pretreatment was used to prepare biomass before enzymatic hydrolysis. The comparison between NaOH, KOH and Ca(OH)2 revealed that NaOH has been more effective on bagasse structure. The required enzymes for biomass hydrolysis were produced by bagasse solid state fermentation using three fungi: Trichoderma longibrachiatum, T. reesei and Aspergillus niger. Results indicated enzyme solution produced by A. niger has functioned better than the other two in cellulose conversion during sole hydrolysis. Ethanol was produced by simultaneous saccharification and fermentation (SSF) with on-site... 

    Investigation of a protein complex network

    , Article European Physical Journal B ; Volume 41, Issue 1 , 2004 , Pages 113-121 ; 14346028 (ISSN) Mashaghi, A. R ; Ramezanpour, A ; Karimipour, V ; Sharif University of Technology
    The budding yeast Saccharomyces cerevisiae is the first eukaryote whose genome has been completely sequenced. It is also the first eukaryotic cell whose proteome (the set of all proteins) and interactome (the network of all mutual interactions between proteins) has been analyzed. In this paper we study the structure of the yeast protein complex network in which weighted edges between complexes represent the number of shared proteins. It is found that the network of protein complexes is a small world network with scale free behavior for many of its distributions. However we find that there are no strong correlations between the weights and degrees of neighboring complexes. To reveal... 

    Treatment of bakers yeast wastewater with a Biopack system

    , Article Process Biochemistry ; Volume 37, Issue 5 , 2001 , Pages 447-451 ; 13595113 (ISSN) Nahid, P ; Vossoughi, M ; Alemzadeh, I ; Sharif University of Technology
    The purpose of this study was to investigate the use of a horizontal packed bed bioreactor, named Biopack, to treat high strength wastewater, such as bakers yeast wastewater. The Biopack system (RBCP) is a modified rotating biological contactor and the system was applied for secondary treatment. The RBCP system produced high treatment efficiencies, COD removal was 94.9% when COD was 472 mg/l and surface organic loading = 0.187 kg COD/m2 per day and surface hydraulic loading = 6 l/m2 per day. Variations of surface hydraulic loading from 6 to 11.9 l/m2 per day produced a decrease in COD removal efficiency from 94.9 to 58.6%. Other factors, such as shock load, retention time and rotational... 

    Production of l-asparaginase from Escherichia coli ATCC 11303: Optimization by response surface methodology

    , Article Food and Bioproducts Processing ; Volume 89, Issue 4 , Oct , 2011 , Pages 315-321 ; 09603085 (ISSN) Kenari, S. L. D ; Alemzadeh, I ; Maghsodi, V ; Sharif University of Technology
    This paper discusses the studies carried out for the optimal production of enzyme l-asparaginase (l-asparagine amidohydrolase, EC from Escherichia coli (ATCC 11303). It was found that inoculum age of 18 h and inoculum size of 10% were the most favorable operating conditions for enzyme production. Lactose, yeast extract and KH 2PO 4 were found to be the best carbon, nitrogen and ion sources, respectively. Statistical method was used to survey how various medium conditions affect the enzyme production. By response surface methodology, the values of lactose, tryptone, yeast extract, KH 2PO 4 and l-asparagine concentration were investigated to obtain the maximum enzyme activity. The... 

    Modeling and control of cell cycle

    , Article 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009 ; 2009 ; 9781424429028 (ISBN) Azizi, A ; Seifipour, N ; Sharif University of Technology
    Applying engineering approaches to non-engineering systems such as biological systems has brought a new research filed to the surface. The sole purpose of this research is mathematical modeling the cell cycle control system in the fissionyeast cell using Ordinary Differential Equations (ODE).This paper, in fact, introduces the capabilities of engineering knowledge and engineering of control, in particular, in this field. ©2009 IEEE  

    Integrated system of multiple batches to evaluate the continuous performance of microbial cells in decolourization processes

    , Article Journal of Environmental Chemical Engineering ; Volume 6, Issue 1 , February , 2018 , Pages 728-735 ; 22133437 (ISSN) Vatandoostarani, S ; Bagheri Lotfabad, T ; Heidarinasab, A ; Ebadipour, N ; Yaghmaei, S ; Sharif University of Technology
    Elsevier Ltd  2018
    Azo dye degradation in wastewater treatment is a subject which has garnered the attention of many research studies. In this study, an innovative approach, namely, an integrated system of five batches (ISFB), was developed to investigate the capability of Saccharomyces cerevisiae ATCC 9763 for continuous degradation of methyl red as a representative azo dye. Toward this end, an expanded immobilized microbial bed (EIMB) reactor was established with a bed of encapsulated yeast cells in sodium alginate. EIMB reactor was run in two modes, single batch and ISFB. Moreover, durability of the microbial cells was evaluated by repeating the continuous decolourization eight sequential times in EIMB at... 

    Comparative assessment of the efficiencies of gas sparging and back-flushing to improve yeast microfiltration using tubular ceramic membranes

    , Article Desalination ; Volume 217, Issue 1-3 , 2007 , Pages 93-99 ; 00119164 (ISSN) Fadaei, H ; Tabaei, S. R ; Roostaazad, R ; Sharif University of Technology
    Gas sparging and back-flushing treatments were compared as a means to tackle the problem of fouling in yeast microfiltration. Based on the feed and membrane characteristics, it was shown that either of these techniques could be superior to the other in order to overcome the problem. At more concentrated feed streams the main cause of flux decline was cake formation. In this condition gas sparging showed greater efficiency in flux enhancement. On the other hand at lower feed concentration the relative importance of internal fouling due to pore blockage, increased. In this case back-flushing was more effective. © 2007  

    Bio alcohol production from agricultural residues

    , Article 3rd International Symposium on Biotechniques for Air Pollution Control, Delft, 28 September 2009 through 30 September 2009 ; 2010 , Pages 167-174 ; 9780415582704 (ISBN) Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    The bioconversion of abundant and renewable cellulosic biomass into ethanol as an alternative to petroleum is gaining importance due to the realization of diminishing natural oil and gas resources. Agricultural and foresty plant residues are an abundant and renewable source of sugar substrates that could be fermented to ethanol. A thermochemical treatment of biomass in which both cellulose and hemicellulose are hydrolyzed to soluble sugar is necessary before yeast fermentation. After thermochemical treatment, cellulase enzymes must be introduced in the system to hydrolyze any remaining cellulose. The simultaneous saccharification and fermentation (SSF), is a method which converts... 

    Parameters Identification for Mathematical Model of The Fission Yeast Cell Cycle Using Genetic Algorithm

    , M.Sc. Thesis Sharif University of Technology Mostafavi Sabet, Azadeh (Author) ; Ghaffari, Ali (Supervisor)
    Soft computing consists of a set of powerful methods that have been successfully applied to many different application domains, including business, engineering, and bioinformatics. In this research we propose an innovative approach that uses genetic algorithms to mine a set of behavior data output by a biological system in order to determine the kinetic parameters of the system. In our approach, the machine learning method is integrated with the framework of existent biological information in a wiring diagram so that its findings are expressed in a form of system dynamic behavior. An application of the method to the cell division cycle model has shown that the method can discover optimum... 

    Extracellular L-Asparaginase Production in Candida Utilis: Production and Activity Determination Conditions

    , M.Sc. Thesis Sharif University of Technology Mahdinia, Ehsan (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Co-Advisor)
    L-Asparaginase has two major uses. For decades it has been known and used as an antitumor agent. Besides, it has been introduced as a food processing aid in the last trench against acrylamide formation in foods. Here, we have produced extracellular L-Asparaginase from Candida utilis and studied the parameters surrounding its production. Thus, two species of yeast were cross-examined: Saccharomyces cerevisiae (BBRC 30006) and Candida utilis (ATCC 9950). Moreover, the most suitable method for detecting and determining L-Asparaginase activity was investigated and selected for this purpose which is via L-Aspartate determination with Hydroxylamine. Furthermore, six deferent parameters of... 

    Optimization of Asparaginase Enzyme in Bioreactor

    , M.Sc. Thesis Sharif University of Technology Momeni, Vahid (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    L-asparaginase enzyme is a well known enzyme due to itschemotherapeutic properties. However, this is not the only feature of the enzyme. L- asparaginase can be also used in food processing to reduce of acrylamide which is a carcinogenic substance. furthermore, acrylamide formation, is prevented by addition of the enzyme before cooking process of some foods such as chips, which contains sugar and asparagine amino acid. Due to use of the enzyme in food industry, we need to produce enzyme with high activity and cheaper raw material. In the present study, Candida utilis ATCC 9950 was used to produce asparaginase enzyme, and beet molasses and yeast extract was used as a carbohydrate and... 

    Rosmarinus officinalis directed palladium nanoparticle synthesis: Investigation of potential anti-bacterial, anti-fungal and Mizoroki-Heck catalytic activities

    , Article Advanced Powder Technology ; Volume 31, Issue 4 , 2020 , Pages 1402-1411 Rabiee, N ; Bagherzadeh, M ; Kiani, M ; Ghadiri, A. M ; Sharif University of Technology
    Elsevier B.V  2020
    The present work was aimed to account a green and eco-friendly synthesis of palladium nanoparticles using Rosmarinus officinalis leaves extracts for the first time, therefore, that can be an acceptable replacement for chemical media to improve potential biological properties. The synthesized palladium nanoparticles were fully characterized using FT-IR, XRD, FESEM, TEM and UV/Vis spectroscopy techniques. Catalytic activity was investigated by Mizoroki-Heck reaction, and optimized based on solvent, temperature and time of the reaction, and the best results were found in water as a green media without any additional reagents. Biological activity of the synthesized nanoparticles were evaluated... 

    Production of extracellular protease and determination of optimise condition by bacillus licheniformis BBRC 100053

    , Article Chemical Engineering Transactions ; Volume 21 , 2010 , Pages 1447-1452 ; 19749791 (ISSN) ; 9788895608051 (ISBN) Ghobadinejad, Z ; Yaghmaei, S ; Haji Hosseini, R ; Sharif University of Technology
    The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1 %, yeast extract 0.5 %, peptone 0.5 %, KH 2PO4 0.1%, MgSO4.7H2O 0.02 %. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35 °C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45 °C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.Thermo stability of the enzyme was considered... 

    Production of extracellular protease and determination of optimal condition by bacillus licheniformis BBRC 100053

    , Article International Journal of Engineering, Transactions B: Applications ; Volume 22, Issue 3 , 2009 , Pages 221-228 ; 1728-144X (ISSN) Ghobadi Nejad, Z ; Yaghmaei, S ; Haji Hosseini, R ; Sharif University of Technology
    Materials and Energy Research Center  2009
    The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO 4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm after 73 hours. Protease activity was highest at pH 8 and 45°C. The best carbon sources are respectively lactose and maltose and the best nitrogen source is peptone. The protease was highly active and stable from pH 7.0 to 11.0 with an optimum at pH 7-8.... 

    Simple, Cost-effective, and continuous 3D dielectrophoretic microchip for concentration and separation of bioparticles

    , Article Industrial and Engineering Chemistry Research ; Volume 59, Issue 9 , 2020 , Pages 3772-3783 Tajik, P ; Saidi, M. S ; Kashaninejad, N ; Nguyen, N. T ; Sharif University of Technology
    American Chemical Society  2020
    Dielectrophoresis is a robust approach for manipulating bioparticles in microfluidic devices. In recent years, many groups have developed dielectrophoresis-based microfluidic systems for separation and concentration of various types of bioparticles, where the gradient of the electric field causes dielectrophoresis force acting on the suspended particles. Enhancing the gradient of the electric field with three-dimensional (3D) electrodes can significantly improve the efficiency of the system. Implementing planar electrodes in a 3D arrangement is a simple option to form a 3D-electrode configuration. This paper reports the development of a novel dielectrophoretic microfluidic system for... 

    Enhancement of bioleaching of a spent Ni/Mo hydroprocessing catalyst by Penicillium simplicissimum

    , Article Separation and Purification Technology ; Volume 80, Issue 3 , August , 2011 , Pages 566-576 ; 13835866 (ISSN) Amiri, F ; Mousavi, S. M ; Yaghmaei, S ; Sharif University of Technology
    Statistically based experimental designs were applied to screen and optimize the bioleaching of spent hydrocracking catalyst by Penicillium simplicissimum. Eleven factors were examined for their significance on bioleaching using a Plackett-Burman factorial design. Four significant variables (pulp density, sucrose, NaNO3, and yeast extract concentrations) were selected for the optimization studies. The combined effect of these variables on metal bioleaching was studied using a central composite design (CCD). Second-order polynomials were established to identify the relationship between the recovery percent of the metals and the four significant variables. The optimal values of the variables... 

    Metabolic Engineering of Yeast Saccharomyces Cerevisiae for Production of Fatty Acids and Its Derivatives

    , M.Sc. Thesis Sharif University of Technology Homayoonfar, Mohammad (Author) ; Roosta Azad, Reza (Supervisor) ; Sardari, Soroush (Supervisor)
    With the increasing emphasis on production of various chemicals by biotechnology industry, the establishment of optimal cell factories for the maximum production of a specific chemical product will be a serious challenge in large-scale production in this industry. Metabolic engineering is a branch of biochemical engineering that has been developed to achieve this and the approaches in it seek to address this challenge. In this study, focusing on increasing production of fatty acids by yeast cells of Saccharomyces cerevisiae, and using a combination of computational and laboratory methods to present a methodology for the optimal design of culture media. Thus, using the constraint-based... 

    Optimization of culture medium and modeling of curdlan production from Paenibacillus polymyxa by RSM and ANN

    , Article International Journal of Biological Macromolecules ; Vol. 70, issue , Jul , 2014 , p. 463-473 Rafigh, S. M ; Yazdi, A. V ; Vossoughi, M ; Safekordi, A. A ; Ardjmand, M ; Sharif University of Technology
    Paenibacillus polymyxa ATCC 21830 was used for the production of curdlan gum for first time. A Box-Behnken experimental design was applied to optimize six variables of batch fermentation culture each at three levels. Statistical analyses were employed to investigate the direct and interactive effects of variables on curdlan production. Optimum cultural conditions were temperature (50. °C), pH (7), fermentation time (96. h), glucose (100. g/L), yeast extract (3. g/L) and agitation speed (150. rpm). The yield of curdlan production was 6.89. g/L at optimum condition medium. Response surface methodology (RSM) and artificial neural network (ANN) were used to model cultural conditions of curdlan... 

    Statistical screening of medium components for recombinant production of Pseudomonas aeruginosa ATCC 9027 rhamnolipids by nonpathogenic cell factory pseudomonas putida KT2440

    , Article Molecular Biotechnology ; Vol. 56, issue. 2 , 2014 , p. 175-191 Setoodeh, P ; Jahanmiri, A ; Eslamloueyan, R ; Niazi, A ; Ayatollahi, S. S ; Aram, F ; Mahmoodi, M ; Hortamani, A ; Sharif University of Technology
    Rhamnolipids (RLs) produced by the opportunistic human pathogen Pseudomonas aeruginosa are considered as potential candidates for the next generation of surfactants. Large-scale production of RLs depends on progress in strain engineering, medium design, operating strategies, and purification procedures. In this work, the rhlAB genes extracted from a mono-RLs-producing strain of P. aeruginosa (ATCC 9027) were introduced to an appropriate safety host Pseudomonas putida KT2440. The capability of the recombinant strain was evaluated in various media. As a prerequisite for optimal medium design, a set of 32 experiments was performed in two steps for screening a number of macro-nutritional... 

    Two-compartment processing as a tool to boost recombinant protein production

    , Article Engineering in Life Sciences ; Vol. 14, issue. 2 , March , 2014 , p. 118-128 Jazini, M ; Herwig, C ; Sharif University of Technology
    Pichia pastoris is used extensively as a production platform for many recombinant proteins. The dissolved oxygen (DO) is one of the most important factors influencing protein production. The influence of the DO on productivity has not been studied independent from the feed rate. In this work, various DO levels were investigated independent from the feed rate. The model system was recombinant P. pastoris under the control of methanol-induced alcohol oxidase promoter, which expressed HRP as the target protein. No significant effect was observed in terms of titer and specific productivity, which is a confirmation of the fact that the DO in a one-compartment system cannot boost productivity for...