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Review on Dye Degradability by Enzymatic Method in a Bioreactor

Mansouri Majoumerd, Mohammad | 2010

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 40296 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Kariminia, Hamid Reza
  7. Abstract:
  8. Effective parameters on decolourization of Sumifix Black B or Reactive Black 5 (RB5) by the extracted peroxidase from Coprinus Cinereus NBRC 30628 in a bioreactor have been reviewed in this master thesis. These parameters were such as hydrogen peroxide concentration, pH of dye solution, enzymatic activity and hydraulic retention time. All of these parameters were optimized in such condition that enzyme solution was added to bioreactor at the initial time of reaction. Optimum concentration for hydrogen peroxide, pH, dye concentration, and hydraulic retention time in above mentioned condition of reactor were 96.1 mM, 8, 40 ppm, and 60 min, respectively. It is worth to note that the higher concentrations of enzyme had led to more efficient decolourization. Then with obtaining the optimum values of significant parameters, the series of continuoes experiments has performed and the dye decolourization percent has reached up to 67.5 percent. However in these series of experiments, enzyme has been added in predetermined time interval. Moreover, the immobilization of enzyme in calcium-alginate system had performed and the resulted immobilizaed enzyme had used in decolourization process. However, the decolourization percent only has reached up to 17 percent and this matter related to the enzyme activity of entrapped enzyme. Moreover, a kinetic model for the rate reaction, based on the Ping-Pong mechanism was assumed for the enzymatic decolourization. Experiments were conducted at different RB5 and hydrogen peroxide concentrations in a batch reactor to estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous tank reactor and the results were compared with experimental results. An acceptable agreement was observed between the model and experiment.

  9. Keywords:
  10. Decolorization ; Peroxidase Enzyme ; Bioreactor ; Coprinus Cinereus Fungi ; Reactive Black 5 ; Bisubstrate Ping-Pong Mechanism

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