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Synthesis and Biological Evaluation of Magnetic Colloids of Ultrasmall Super Paramgnetic Iron Oxide Nanoparticles for Biomedical Applications

Abedini, Fatemeh | 2010

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 41345 (07)
  4. University: Sharif University of Technology
  5. Department: Materials Science and Engineering
  6. Advisor(s): Maddah Hosseini, Hamid Reza; Shokrgozar, Mohammad Ali
  7. Abstract:
  8. Present study is designed to determine the effect of manganese content in primitive inverse spinel structure of iron oxide nanoparticles. PEG coated water base ferrofluids of Mnx¬Fe3-xO4 (0<0>1)nanoparticles were prepared by co-precipitation method in presence of nitrogen gas and ultrasonic waves using NaOH as a base for biomedical purposes. The crystallite size and structure of synthesized nanoparticles were studied using atomic absorption spectroscopy (AAS), X-ray diffraction spectrometry (XRD), field emission scanning electron microscopy (FE-SEM) and dynamic light scattering (DLS) methods. Thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR) were used to investigate the surface properties of coated iron oxide nanoparticles. The magnetic characteristics of prepared ferrofluids were measured using vibrating sample magnetometer (VSM) technique. Finally, the in-vitro biocompatibility of synthesized nanoparticles was studied by MTT assay testing. Furthermore, the Prussian blue staining used for particle detection. The results indicated that adding manganese to spinel structure do not have an obvious effect on the size of synthesized nanoparticles. In addition, nanoparticles prepared in all conditions were quasi-spherical and had a narrow size distribution. Increased mole fraction of manganese amount till 0.6, results in improved magnetic properties. The sample with 0.4 mole fraction of manganese had the best magnetic properties. MTT assay showed dose-dependent cell viability for ferrite ferrofluids. Sample with 0.4 mole fraction of manganese and 0.25 mg/ml concentration had a high biocompatibility in 48h which is suitable for biomedical applications
  9. Keywords:
  10. Ferrofluid ; Iron Oxide ; Manganese ; Co-Precipitation ; Biocompatibility

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