Separation and Purification of Industrial Proteins by Chromatography and Aqueous two Phase System, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Seifkordi, Ali Akbar (Supervisor)
Abstract
Separation of biomolecules such as enzymes is the most important part of biotechnology process which 10-90 % of costs is for separation and purification. There are several methods for purification of bio materials and one of them is chromatography which use in enzyme purification but this method have several problem such as cost ,time consuming and scale up Because of this a suitable alternative demands for industrial separation. An alternative is aqueous two-phase systems (ATPS). In this project was studied about partial purification of alkaline protease produced by Bacillus licheniformis ATCC 21424 in aqueous two-phase system and comparison with chromatography method. an aqueous two-phase...
Cataloging briefSeparation and Purification of Industrial Proteins by Chromatography and Aqueous two Phase System, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Seifkordi, Ali Akbar (Supervisor)
Abstract
Separation of biomolecules such as enzymes is the most important part of biotechnology process which 10-90 % of costs is for separation and purification. There are several methods for purification of bio materials and one of them is chromatography which use in enzyme purification but this method have several problem such as cost ,time consuming and scale up Because of this a suitable alternative demands for industrial separation. An alternative is aqueous two-phase systems (ATPS). In this project was studied about partial purification of alkaline protease produced by Bacillus licheniformis ATCC 21424 in aqueous two-phase system and comparison with chromatography method. an aqueous two-phase...
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