Design and Construction of Alginate-Silica Nanocomposite and Its Application for Mammalian Cell Culture

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Mohammadi Fard, Hossein | 2011

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 42506 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Vossughi, Manouchehr; Arpanaei, Ayyoob; Kabir Salmani, Maryam
Abstract:
Three sizes of silica nanoparticles (160±7, 450±10 and 600±25) were sythetized via template-removing method. The synthetized nanoparticles were functionalized with N-(2-aminoethyl)-3-aminopropyl trimethoxy-silane (EDS) to provide a positive charge on the surface of nanoparticles. BET analysis showed that the average pore diameter, surface area and pore volume of nanoparticles were 2.3 nm, 769 m2.g-1 and 0.44 cm3.g-1, respectively. showed that the Amine-functionalized silica nanoparticles were used to enhance the application of alginate polymer for 3D encapsulation of mammalian cells. Incorporating of silica nanoparticles can be suitable for the bead integrity and attachement of mammalian cells. The obtained results revealed that amine-functionalized silica nanoparticles-incorporated alginate beads had no negetive effect on the CHO cell density and viability compared to that in the bare alginate beads
Keywords:
Nanocomposite ; Silica Nanoparticles ; Template Removing ; Alginate Beads ; Ukaryotic Cells Culture

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