Kinetics of Animal Cell Adhesion Mediated by the Diffusion of Cell Wall Receptors: Effect of Mechanical Stiffness and Electrical Charges of the Substrate

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Samadi Dooki, Aref | 2012

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 42625 (09)
University: Sharif University of Technology
Department: Civil Engineering
Advisor(s): Mohammadi Shoja, Hossein
Abstract:
Viability and proliferation of cells in cell culture mainly depends on i) cell culture medium, ii) cell-cell interaction, and iii) the supporting scaffold. Among these factors, and within recent years, surface and mechanical properties of the supporting ECM has attracted many researchers. Experiment results show that the animal cell adhesion is accelerated on stiffer scaffolds. Electrical charge of the scaffold is also a determinant term in the kinetics of adhesion and on a relatively charged scaffold, one can expect an increment in kinetics of adhesion front movement. This is due to the natural negative charges of the cell wall membrane. In this thesis, an analytical model is proposed to model the mechanics of animal cell adhesion. This phenomenon is mediated by the diffusion of cell wall receptor proteins and controlled by the stiffness and electrical charges of the substrate. The results support the affinity of cell to adhere to a relatively stiffer scaffold. In addition, the increment in kinetics of adhesion front movement by the increase in charges of the substrate is also carried out
Keywords:
Ligands ; Lipid Bilayer Membrane ; Cell Adhesion ; Focal Adhesion ; Cell Wall Receptors ; Extracellular Matrix