Loading...

Synthesis of Cationic Mesoporous Silica Nanoparticles as a Carrier for the Deliveryof Nucleic Acids

Kermanshah, Leyla | 2012

1165 Viewed
  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 42885 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Vosoughi, Manouchehr; Arpanaei, Ayyoob; Javadi, Hamid Reza
  7. Abstract:
  8. Mesoporous silica nanoparticles (MSNP) have attracted lots of attentions because of their particular characteristics. Physical characteristics such as structure, morphology, porosity, and size of these nanoparticles have strong impact on their function and it can be conceived a wide range of applications for them by manipulating these characteristics. In this research, monodispersed MSNPs with a controllable size in the range of 50-130 nm and pore size in the range of 4-24 nm were synthesized and positively functionalized in order to develop a carrier for the delivery of nucleic acids (siRNA and pDNA). The MSNPs were synthesized by the template removing method.In this method, sodium hydroxide (NaOH) and cetyl trimethyl ammonium bromide (CTAB) were used as the catalyst and template-forming surfactant, respectively. The pores were expanded through calcination in the presence of complex salts. During the synthesis of MSNP, the effect of stirrer’s rotational rate and concentration of tetraethylorthosilicate (TEOS), as the silica precursor, were investigated. The effect of calcination temperature was observed during the pore expansion process. MSNPs’ surface was functionalized with a cationic functionality and DNA adsorption behavior was investigated. MSNPs were characterized for the size, morphology, pore size, and charge.The DNAadsorption behavior is studied and the results revealed that the MSNPs with larger pore size, despite of lower zeta potential and specific surface area, have higher values of DNA binding.
  9. Keywords:
  10. Template Removing ; Functionalization ; Mesoporous Silica Nanoparticles ; Pore Size ; DNA Adsorption ; Nucleic Acid Delivery

 Digital Object List

  • محتواي پايان نامه
  •   view

 Bookmark

No TOC