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Spectroscopic Study and Characterizing of Selenium Dioxide Complex with Pyridoxine(PN-Se):Interaction Between (PN-Se)and Bovine Serum Albumin

Jamali, Moein | 2012

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 43811 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Mohamadi Boghaei, Davar
  7. Abstract:
  8. Temporary existence of some molecules like medicines in body and also existence of diagnostic agents in biological systems and study of the interactions of biomolecules with these agents is a matter of interest. It also may have an important role in design and synthesis of new drugs. Serum Albumin is the most abundant protein in circulation system in the body. So far, many studies about the interaction of this protein with different compounds have been undertaken. The aim of this paper was to synthesize selenium complex and analyze its potential biochemical activity in vitro. The concrete work falls into two parts:(1) to synthesize the selenium-pyridoxine compound (PNSe) (2) to probe into the interaction between (PNSe) and BSA under simulated physiological conditions (ionic strength=0.1 mol•Lˉ1; pH 7.40).
    for this purposes a selenium-pyridoxine complex (PNSe) was synthesized and its structure was identified by IR, CHN,1H-NMR and UV-VIS.The interaction of (PNSe) and BSA was discussed by fluorescence quenching method and Förster non-radiation energy transfer theory. The thermodynamic parameters ΔHθ,ΔGθ, ΔSθ at different temperatures were calculated and the results indicated the interaction was a spontaneous as well as an endothermic process. In this paper, a coordination compound with molar ratio of 1:1 (pyridoxine:Se) has formed .Under simulated physiological conditions in vitro, the synthesized compound (PNSe) can bind to BSA mainly at ground state, which leads to static fluorescence quenching in BSA molecules, with non-radiation energy transfer happened within single molecule. The next step of our work is to examine the pharmacologic activity of (PNSe) in vivo, which aims at offering evidence in support of the possibility to develop it as pharmaceuticals for diseases relating to the lack of selenium
  9. Keywords:
  10. Quenching ; Bovine Serum Albumin (BSA) ; Selenium-Pyridoxine ; Forster Nonradiation Energy Transfer Theory ; Fluorescence Quenching

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