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Feasibility Study of Culturing and Differentiating Mesenchymal Stem Cells to Smooth Muscle Cells in Nanostructured PCL Hybrid Scaffold

Moghadasi, Samaneh | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45522 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s):
  7. Abstract:
  8. Tissue engineering is an interdisciplinary field that incorporates principles of engineering with the life sciences. Tissue engineering consists of three principle; cells, scaffolds for cells expansion and growth factors. These factors together can help tissue engineers to provide appropriate microenvironments for directing special cells behavior.
    Fibrous scaffolds have found many applications in tissue engineering due to their nanometer dimensions and high surface to volume ratio. Fibrous scaffolds can impart mechanical strength, structure for cell attachment and act as reservoirs for biomolecule delivery in much a same way as the natural fibrous components of the extracellular matrix.
    In this study feasibly of differentiating ofumbilical cord Wharton’s jelly mesenchymal stem cells to bladder smooth muscle cells was investigated. To achieve this goal, the effects of nanostructure PCL/PLLA scaffold topography as well as control release of Transforming Growth Factor β1 were investigated.Real time PCR results show that nanostructure PCL/PLLA scaffold topography together with ascorbic acid and control release TGF-β1 plays an important role in differentiation of umbilical Wharton’s jelly mesenchymal stem cells to smooth muscle cells
  9. Keywords:
  10. Mesenchymal Stem Cell Diffentiation ; Nanostructured Scaffold ; Bladder Tissue Engineering

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