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Numerical Simulation of Shaking Bioreactors for Cell Culture

Ghahremannezhad, Ali | 2014

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 45626 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Saidi, Mohammad Said
  7. Abstract:
  8. A bioreactor can refer to any device or system constructed on engineering basis and has an active biological medium. This medium includes aerobic and non-aerobic reactions in which organisms and the theire produced substances have the main role. One of the applications of bioreactors is to provide an appropriate medium for animal and plant cell culture and growth. Nowadays many biological processes in high volume scales are carried out in stirred tank bioreactors. In these bioreactors oxygen transfer is usually increased by increasing turbulence which is necessary for mixing nutrients and keeping the homogeneity of the medium. However, there are some limitations for increasing the stirring speed due to high power consumption and possible damage to cells. In recent years, shaking bioreactors have been introduced as suitable bioreactors beside the stirred tanks. Yet, there is limited understanding about the engineering characteristics of these type of bioreactors like fluid dynamics and mass transfer.
    In this work, first the hydrodynamics of the multiphase fluid flow inside a shaking bioreactor is simulated by Computational Fluid Dynamics with the Volume of Fluid method to better understand the fluid flow patterns in shaking bioreactors. The Volume of Fluid equations are solved explicitly and to track the free surface, Geo-Reconstruct method is used. PISO algortithm is used to solve the coupled pressure and velocity equations. The CFD equations were solved numerically by ANSYS FLUENT software. The bioreactor movement is simulated by Dynamic Mesh. Afterwards, the oxygen transfer coefficient in shaking bioreactors is calculated and mass transfer is investigated by using some User Defined Functions.
  9. Keywords:
  10. Computational Fluid Dynamics (CFD) ; Oxygen Transfer ; Volume of Fluid ; Numerical Simulation ; Cell Culture ; Shaking Bioreactors

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