Design and Fabrication of Scaffold Composed of Acellular Cartilage Matrix and Chitosan

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Sivandzade, Farzane | 2015

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 47591 (06)
University: Sharif University of Technology
Department: Chemical and Petroleum Engineering
Advisor(s): Mashayekhan, Shohreh; Hasanzadeh, Zabihollah
Abstract:
Cartilage is an avascular tissue, having limited ability to repair itself. Since the methods for treatment of cartilage defects have been not effective, in recent years, new therapies based on tissue engineering are considered.This paper reports on the development of porous microcarriers composed of acellular matrix of cartilage and natural polymer chitosan. Microcarriers were prepared by electrospray method. Results of mechanical tests, SEM imaging, water uptake behaviour, biodegradation test, and MTT assay demonstrated that the microcarriers composed of 2% (wt) chitosan and 1% (wt) ECM has the best potential for growth and proliferation of primary chondrocyte cells. These results demonstrated that the aforementioned microcarriers have good potential to be used for cartilage tissue engineering applications
Keywords:
Cartilage Tissue Engineering ; Extracellular Matrix ; Chitosan ; Decellularization ; Microcarrier

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