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Efficient biodegradation of naphthalene by a newly characterized indigenous achromobacter sp. FBHYA2 isolated from Tehran oil refinery complex

Farjadfard, S ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.2166/wst.2012.217
  3. Publisher: IWA Pub , 2012
  4. Abstract:
  5. A bacterial strain, FBHYA2, capable of degrading naphthalene, was isolated from the American Petroleum Institute (API) separator of the Tehran Oil Refinery Complex (TORC). Strain FBHYA2 was identified as Achromobacter sp. based on physiological and biochemical characteristics and also phylogenetic similarity of 16S rRNA gene sequence. The optimal growth conditions for strain FBHYA2 were pH 6.0, 30°C and 1.0% NaCl. Strain FBHYA2 can utilize naphthalene as the sole source of carbon and energy and was able to degrade naphthalene aerobically very fast, 48 h for 96% removal at 500 mg/L concentration. The physiological response of Achromobacter sp., FBHYA2 to several hydrophobic chemicals (aliphatic and aromatic hydrocarbons) was also investigated. No biosurfactant was detected during bacterial growth on any aliphatic/aromatic hydrocarbons. The results of hydrophobicity measurements showed no significant difference between naphthalene- and LB-grown cells. The capability of the strain FBHYA2 to degrade naphthalene completely and rapidly without the need to secrete biosurfactant may make it an ideal candidate to remediate polycyclic aromatic hydrocarbon (PAH)-contaminated sites
  6. Keywords:
  7. Biosurfactant ; 16S rRNA gene sequence ; American Petroleum Institute ; Bio surfactant ; Biochemical characteristics ; Contaminated sites ; Hydrophobic chemicals ; Hydrophobicity measurement ; Oil refineries ; Optimal growth ; Physiological response ; Sole source of carbon ; Surface hydrophobicity ; Biomolecules ; Hydrophobicity ; Microbiology ; Petroleum refineries ; Physiological models ; Polycyclic aromatic hydrocarbons ; RNA ; Sodium chloride ; Surface active agents ; Aliphatic hydrocarbon ; Aromatic hydrocarbon ; Bacterium ; Carbon ; Energy ; Hydrophobicity ; Naphthalene ; Oil industry ; Oxic conditions ; PAH ; PH ; Phylogenetics ; Physiology ; Surfactant ; Aerobic metabolism ; Bacterial growth ; Bacterial strain ; Bacterium isolation ; Biodegradation ; Carbon source ; Concentration (parameters) ; Controlled study ; Energy resource ; Nonhuman ; Nucleotide sequence ; Achromobacter ; Biodegradation, Environmental ; Cell Membrane ; Hydrocarbons, Aromatic ; Hydrophobic and Hydrophilic Interactions ; Iran ; Kinetics ; Naphthalenes ; Oil and Gas Fields ; Petroleum ; RNA, Ribosomal, 16S ; Surface-Active Agents ; Achromobacter sp ; Bacteria (microorganisms)
  8. Source: Water Science and Technology ; Volume 66, Issue 3 , March , 2012 , Pages 594-602 ; 02731223 (ISSN)
  9. URL: http://wst.iwaponline.com/content/66/3/594