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Amperometric sulfide detection using Coprinus cinereus peroxidase immobilized on screen printed electrode in an enzyme inhibition based biosensor

Savizi, I. S. P ; Sharif University of Technology | 2012

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  1. Type of Document: Article
  2. DOI: 10.1016/j.bios.2012.03.004
  3. Publisher: 2012
  4. Abstract:
  5. In the present work, an amperometric inhibition biosensor for the determination of sulfide has been fabricated by immobilizing Coprinus cinereus peroxidase (CIP) on the surface of screen printed electrode (SPE). Chitosan/acrylamide was applied for immobilization of peroxidase on the working electrode. The amperometric measurement was performed at an applied potential of -150. mV versus Ag/AgCl with a scan rate of 100. mV in the presence of hydroquinone as electron mediator and 0.1. M phosphate buffer solution of pH 6.5. The variables influencing the performance of sensor including the amount of substrate, mediator concentration and electrolyte pH were optimized. The determination of sulfide can be achieved in a linear range of 1.09-16.3 μM with a detection limit of 0.3 μM. Developed sensor showed quicker response to sulfide compared to the previous developed sulfide biosensors. Common anions and cations in environmental water did not interfere with sulfide detection by the developed biosensor. Cyanide interference on the enzyme inhibition caused 43.25% error in the calibration assay which is less than the amounts reported by previous studies. Because of high sensitivity and the low-cost of SPE, this inhibition biosensor can be successfully used for analysis of environmental water samples
  6. Keywords:
  7. Coprinus cinereus peroxidase ; Enzyme inhibition biosensor ; Screen printed electrode ; Sulfide detection ; Ag/AgCl ; Amperometric ; Amperometric measurements ; Applied potentials ; Coprinus cinereus ; Detection limits ; Electron mediator ; Environmental water ; Environmental water samples ; High sensitivity ; Linear range ; Phosphate buffer solutions ; Scan rates ; Screen printed electrodes ; Working electrode ; Cyanides ; Ketones ; Phenols ; Sensors ; Sulfur compounds ; Biosensors ; Acrylamide ; Chitosan ; Cyanide ; Hydroquinone ; Peroxidase ; Silver ; Silver chloride ; Sulfide ; Amperometric biosensor ; Analytical error ; Calibration ; Controlled study ; Coprinus ; Electrode ; Enzyme immobilization ; Enzyme inhibition ; Limit of detection ; PH ; Biosensing Techniques ; Electrochemical Techniques ; Electrodes ; Enzymes, Immobilized ; Fungal Proteins ; Hydrogen Peroxide ; Hydrogen-Ion Concentration ; Hydroquinones ; Peroxidases ; Reproducibility of Results ; Sulfides ; Water Pollutants, Chemical ; Coprinopsis cinerea
  8. Source: Biosensors and Bioelectronics ; Volume 35, Issue 1 , 2012 , Pages 297-301 ; 09565663 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0956566312001455