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Enhancement of L-asparaginase production by candida utilis in a 13L fermenter and its purification

Momeni, V ; Sharif University of Technology | 2015

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  1. Type of Document: Article
  2. Publisher: Materials and Energy Research Center , 2015
  3. Abstract:
  4. L- asparaginase enzyme is a renown enzyme because of its chemotherapeutic properties. This enzyme may also be employed in food processing technology. The present study aimed, optimizing the agitation and aeration rate in L-asparaginase production, using C. utilis, ATCC 9950 in batch fermentation system. Beet molasses used as the carbohydrate source for enzyme production. A maximum asparaginase activity of 245.6 U/ml obtained after 20h of fermentation under optimal condition of 1.25 vvm aeration rate and 300 rpm agitation speed. Purification of the enzyme was also done by Acetone Precipitation and column chromatograph. Purified L-asparaginase revealed molecular weight 75 kDa and 82.7 % yield with 10.02 fold increase in specific activity
  5. Keywords:
  6. Aeration Rate ; Agitation Speed ; Asparaginase ; C. utilis ; Purification ; Acetone ; Enzymes ; Fermentation ; Food processing ; Acetone precipitations ; Carbohydrate sources ; Food processing technology ; Optimal conditions
  7. Source: International Journal of Engineering, Transactions B: Applications ; Volume 28, Issue 8 , 2015 , Pages 1133-1141 ; 1728-144X (ISSN)
  8. URL: http://www.civilica.com/Paper-JR_IJE-JR_IJE-28-8_004=Enhancement-of-L-asparaginase-Production-by-Candida-utilis-in-a-13-L-Fermenter-and-its-Purification.html