Loading...

A colorimetric sensor array for detection and discrimination of biothiols based on aggregation of gold nanoparticles

Ghasemi, F ; Sharif University of Technology | 2015

2445 Viewed
  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2015.04.011
  3. Publisher: Elsevier , 2015
  4. Abstract:
  5. Developments of sensitive, rapid, and cheap systems for identification of a wide range of biomolecules have been recognized as a critical need in the biology field. Here, we introduce a simple colorimetric sensor array for detection of biological thiols, based on aggregation of three types of surface engineered gold nanoparticles (AuNPs). The low-molecular-weight biological thiols show high affinity to the surface of AuNPs; this causes replacement of AuNPs' shells with thiol containing target molecules leading to the aggregation of the AuNPs through intermolecular electrostatic interaction or hydrogen-bonding. As a result of the predetermined aggregation, color and UV-vis spectra of AuNPs are changed. We employed the digital mapping approach to analyze the spectral variations with statistical and chemometric methods, including hierarchical cluster analysis (HCA) and principal component analysis (PCA). The proposed array could successfully differentiate biological molecules (e.g., cysteine, glutathione and glutathione disulfide) from other potential interferences such as amino acids in the concentration range of 10-800μmolL-1
  6. Keywords:
  7. Aggregation ; Gold nanoparticle ; Agglomeration ; Amino acids ; Biomolecules ; Chromatography ; Color ; Colorimetry ; Fiber optic sensors ; Hierarchical systems ; Hydrogen bonds ; Molecular biology ; Molecules ; Nanoparticles ; Peptides ; Photomapping ; Biothiol ; Chemometric method ; Detection and discriminations ; Intermolecular electrostatic interaction ; Potential interferences ; Principal component analysis ; Biothiol derivative ; Cysteine ; Glutathione ; Glutathione disulfide ; Sodium chloride ; Thiol derivative ; Unclassified drug ; Binding affinity ; Chemical analysis ; Cluster analysis ; Colorimetric sensor array ; Concentration (parameters) ; Controlled study ; Hierarchical cluster analysis ; Human ; Human cell ; Light scattering ; Material coating ; Molecular weight ; PH ; Physical parameters ; Priority journal ; Static electricity ; Surface property ; Synthesis ; Transmission electron microscopy ; Ultraviolet spectrophotometry ; Devices ; Gold ; Hydrogen-Ion Concentration ; Metal Nanoparticles ; Microscopy, Electron, Transmission ; Spectrophotometry, Ultraviolet ; Sulfhydryl Compounds
  8. Source: Analytica Chimica Acta ; Volume 882 , July , 2015 , Pages 58-67 ; 00032670 (ISSN)
  9. URL: http://www.sciencedirect.com/science/article/pii/S0003267015004766