Investigation of Activation Mechanism of Mechanosensitive Nano Ion Channels, Ph.D. Dissertation Sharif University of Technology ; Vosoughi, Manochehr (Supervisor) ; Jamali, Yusef (Supervisor) ; Naghdabadi, Reza (Supervisor)
Abstract
Mechanosensitive (MS) channels are ubiquitous molecular force sensors that respond to a number of different mechanical stimuli including tensile, compressive and shear stress. One of the main mechanisms to functionally study these channels is the patch clamp technique. Here we use continuum mechanics to probe the question of how curvature, in a standard patch clamp experiment, at different length scales (global and local) affects a model MS channel. Firstly, to increase the accuracy of the Laplace’s equation in tension estimation in a patch membrane and to be able to more precisely describe the transient phenomena happening during patch clamping, we propose a modified Laplace’s equation. In...
Cataloging briefInvestigation of Activation Mechanism of Mechanosensitive Nano Ion Channels, Ph.D. Dissertation Sharif University of Technology ; Vosoughi, Manochehr (Supervisor) ; Jamali, Yusef (Supervisor) ; Naghdabadi, Reza (Supervisor)
Abstract
Mechanosensitive (MS) channels are ubiquitous molecular force sensors that respond to a number of different mechanical stimuli including tensile, compressive and shear stress. One of the main mechanisms to functionally study these channels is the patch clamp technique. Here we use continuum mechanics to probe the question of how curvature, in a standard patch clamp experiment, at different length scales (global and local) affects a model MS channel. Firstly, to increase the accuracy of the Laplace’s equation in tension estimation in a patch membrane and to be able to more precisely describe the transient phenomena happening during patch clamping, we propose a modified Laplace’s equation. In...
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