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Evaluation of a recycling bioreactor for biosurfactant production by Pseudomonas aeruginosa MR01 using soybean oil waste

Bagheri Lotfabad, T ; Sharif University of Technology

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  1. Type of Document: Article
  2. DOI: 10.1002/jctb.4733
  3. Publisher: John Wiley and Sons Ltd
  4. Abstract:
  5. This study deals with the large-scale production of biosurfactant from soybean oil soapstock by Pseudomonas aeruginosa MR01. The production of biosurfactant was carried out in a newly designed bioreactor equipped with recycling flow under three operational conditions. Kinetic studies were conducted at both shake flask and 5-L bioreactor scales during fermentation in a soapstock medium. Mathematical equations were developed to model the kinetic patterns at both scales. RESULT: Statistical analyses demonstrated the goodness of fit, with regression r-squared, R2, between 0.97and 0.99 for different models. Furthermore, biosurfactant concentration in the bioreactor including the recycling flow, which was switched on and off during a 6-h period, reached 25.5 g L-1 (YP /S=0.9) which showed 41.5% and 21.5% growth compared with experiments conducted in a shake flask and a non-recycling-flow bioreactor, respectively. Moreover, the crude biosurfactant at a concentration of 24 mg L-1 was observed to have lowered the surface tension of double-distilled water from 72 mN m-1 to 28.5 mN m-1 and displayed an emulsification index (E24) of 63% against kerosene. CONCLUSION: The results showed the recycling-flow bioreactor to be a feasible model for further large-scale studies of biosurfactants
  6. Keywords:
  7. Green engineering/products (rhamnolipid) ; Mathematical modeling ; Bacteria ; Bioconversion ; Biological water treatment ; Biomolecules ; Bioreactors ; Bottles ; Emulsification ; Enzyme kinetics ; Fermentation ; Kinetics ; Mathematical models ; Recycling ; Surface active agents ; Biosurfactant production ; Double distilled water ; Emulsification index ; Large scale productions ; Mathematical equations ; Operational conditions ; Pseudomonas aeruginosa ; Rhamnolipids ; Soybean oil
  8. Source: Journal of Chemical Technology and Biotechnology ; Volume 91, Issue 5 , 2016 , Pages 1368-1377 ; 02682575 (ISSN)
  9. URL: http://onlinelibrary.wiley.com/doi/10.1002/jctb.4733/abstract