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Phenols removal by immobilized horseradish peroxidase

Alemzadeh, I ; Sharif University of Technology | 2009

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  1. Type of Document: Article
  2. DOI: 10.1016/j.jhazmat.2008.12.026
  3. Publisher: 2009
  4. Abstract:
  5. Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Increasing initial phenol concentration results in a decrease in % conversion. The highest conversion belongs to phenol concentration of 2 mM. Investigation into time course of phenol removal for both encapsulated and free enzymes showed that encapsulated enzyme had lower efficiency in comparison with the same concentration of free enzyme; however the capsules were reusable up to four cycles without any changes in their retention activity. Increasing enzyme concentration from 0.15 to 0.8 units/g alginate results in gradual increase in phenol removal. The ratio of hydrogen peroxide/phenol at which highest phenol removal obtained is found to be dependent on initial phenol concentration and in the solution of 2 and 8 mM phenol it was 1.15 and 0.94 respectively. © 2008 Elsevier B.V. All rights reserved
  6. Keywords:
  7. Calcium alginate ; Encapsulation ; Ca alginates ; Concentration of ; Encapsulated enzymes ; Enzyme concentrations ; Four cycles ; Free enzymes ; Horseradish peroxidase (HRP) ; Immobilized horseradish peroxidase ; Optimal conditions ; Phenol concentrations ; Phenol removals ; Phenols removals ; Sodium alginates ; Time course ; Alginate ; Calcium ; Calcium alloys ; Calcium chloride ; Coagulation ; Computer software reusability ; Concentration (process) ; Enzyme immobilization ; Enzymes ; Gelation ; Hydrogen ; Oxidation ; Sodium ; Phenols ; Alginic acid ; Phenol derivative ; Enzyme activity ; Immobilization ; PH ; Phenol ; Pollutant removal ; Acidity ; Alkalinity ; Article ; Alginates ; Enzymes, Immobilized ; Horseradish Peroxidase ; Hydrogen-Ion Concentration ; Kinetics ; Armoracia rusticana ; Hydrogen peroxide
  8. Source: Journal of Hazardous Materials ; Volume 166, Issue 2-3 , 2009 , Pages 1082-1086 ; 03043894 (ISSN)
  9. URL: https://www.ncbi.nlm.nih.gov/pubmed/19144465