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Multifunctional core-shell nanoplatforms (gold@graphene oxide) with mediated NIR thermal therapy to promote miRNA delivery

Assali, A ; Sharif University of Technology | 2018

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  1. Type of Document: Article
  2. DOI: 10.1016/j.nano.2018.05.016
  3. Publisher: Elsevier Inc , 2018
  4. Abstract:
  5. Recent insights into the nanomedicine have revealed that nanoplatforms enhance the efficacy of carrier in therapeutic applications. Here, multifunctional nanoplatforms were utilized in miRNA-101 delivery and NIR thermal therapy to induce apoptosis in breast cancer cells. Au nanorods (NRs) or nanospheres (NSs) covered with graphene oxide (GO) were prepared and functionalized with polyethylene glycol as a stabilizer and poly-L-arginine (P-L-Arg) as a targeting agent. In nanoplatforms, coupling Au@GO prepared stable structures with higher NIR reactivity. P-L-Arg substantially enhanced the cellular uptake and gene retardation of stuffs coated by them. However, rod-shape nanoplatforms indicated better performance in cellular uptake and gene transfection than spherical ones. NIR thermal therapy was implemented to improve gene release and in synergy with miRNA-101 activated the apoptotic pathway and decreased the viability of breast cancer cell (<20%). Briefly, presented delivery systems are potentially efficient in distinguishing cancer cells, miRNA internalization and controlling apoptosis of cancer cells. © 2018 Elsevier Inc
  6. Keywords:
  7. Gold nanoplatform ; Graphene oxide ; MiRNA ; Poly-L-arginine ; Amino acids ; Arginine ; Cell death ; Diseases ; Gene therapy ; Gold compounds ; Graphene ; Infrared devices ; Medical nanotechnology ; Molecular biology ; Nanorods ; Positive ions ; Apoptotic pathways ; Breast cancer cells ; L-Arginine ; Photothermal therapy ; Stable structures ; Therapeutic Application ; Thermal therapies ; RNA ; Gold nanorod ; Graphene oxide ; Macrogol ; MicroRNA 101 ; Nanosphere ; Polyarginine ; Apoptosis ; Article ; Atomic force microscopy ; Breast cancer ; Cancer cell ; Cancer gene therapy ; Carboxylation ; Cell viability ; Cellular distribution ; Comparative study ; Cytotoxicity ; Decomposition ; Female ; Flow cytometry ; Gene delivery system ; Gene expression ; Genetic transfection ; Human ; Human cell ; MCF-7 cell line ; MDA-MB-231 cell line ; Molecular weight ; MTT assay ; Nanomedicine ; Real time polymerase chain reaction ; Static electricity ; Surface property ; Thermography ; Thermogravimetry ; Transmission electron microscopy ; Ultraviolet visible spectroscopy
  8. Source: Nanomedicine: Nanotechnology, Biology, and Medicine ; Volume 14, Issue 6 , 2018 , Pages 1891-1903 ; 15499634 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/pii/S1549963418301084