Enzymes, which are biochemical catalysts, have important applications in different industries such as food, pharmaceutical, medical, chemical identification and etc. Several methods have been used for separation of enzymes among which precipitation, chromatography and two-phase system. In this work, purification of α-amylase has been done by Ammonium Sulfate and Acetone precipitation, gel filtration based on Sephadex, and PEG-salt aqueous two-phase system. Furthermore, mass and momentum conservations’ equations were solved simultaneously to simulate the process based on the finite element method (FEM). Crude enzyme was precipitated with different saturations of ammonium sulfate and the...
Enzymes, which are biochemical catalysts, have important applications in different industries such as food, pharmaceutical, medical, chemical identification and etc. Several methods have been used for separation of enzymes among which precipitation, chromatography and two-phase system. In this work, purification of α-amylase has been done by Ammonium Sulfate and Acetone precipitation, gel filtration based on Sephadex, and PEG-salt aqueous two-phase system. Furthermore, mass and momentum conservations’ equations were solved simultaneously to simulate the process based on the finite element method (FEM). Crude enzyme was precipitated with different saturations of ammonium sulfate and the...