Design and Fabrication of a Microfluidic Digital PCR

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Bahrami, Mohammad Amin | 2019

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Type of Document: M.Sc. Thesis
Language: Farsi
Document No: 51594 (08)
University: Sharif University of Technology
Department: Mechanical Engineering
Advisor(s): Saeedi, Mohammad Saeed; Firouzabadi, Bahar
Abstract:
Polymerase chain reaction, abbreviated as PCR, is a method of amplifying the number of copies of a desired DNA sequence through special protocols. The rapid advance of microfluidic devices and the emerging concept of digital microfluidics has resulted into the development of digital droplet PCR (ddPCR) systems with their significant uses in the detection of rare mutations, cancer diagnosis, and surveillance. In this study, an integrated ddPCR microfluidic chip alongside its thermoelectric thermal cycler and optical analysis systems have been designed, fabricated and their performance has been tested and evaluated. It was shown that the effect of master mold’s surface quality, the material of the microchannel and the grade of continuous phase mineral oil is substantial to final results and finally, suggestions were made to be implemented in future studies for designing and manufacturing more robust and easy-to-use systems
Keywords:
Microfluidic System ; Domino Reaction ; DNA Sequencing ; Circulating Tumor Cells (CTC) ; Digital Droplet Polymerase Chain Reaction (PCR)

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