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CTC Cluster Separation on Microfluidics Chip

Rostami, Peyman | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51762 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Firouzabadi, Bahar; Saidi, Mohammad Said
  7. Abstract:
  8. According to statistics in 2012, around 14 million people worldwide have cancer, and 8 million have died that year, according to estimates, this number will increase to 21 millions new cancer case and 13 million mortality in 2030. Despite years of research on cancer, many questions about the mechanism of the spread of cancer in the body remain unanswered so far. The proposed models identify that the cells isolated from the primary tumor and transmit them to the circulatory system in the form of circulating tumor cells (CTCs) or in the form of clusters as responsible for the incidence of cancer. This process is called metastasis. Recent studies have reported the high potential of metastasis by cancerous clusters compared to single CTCs. The exact mechanism of this phenomenon remains hidden so far. In recent years, several methods have been developed based on the physical, chemical, biological, etc. properties difference of CTCs and other blood cells to identify, isolate and count single CTCs in the form of active and passive techniques. However, the proposed techniques have not been shown to be suitable for isolating very rare CTC clusters in the blood, and to this day, little research has been conducted on clusters. In the present study, the goal is to optimize the microchannel spiral with the stair-like geometry in order to achieve a high-performance method for separating CTC clusters from the blood sample. Initially, the efficiency of this method was evaluated numerically, using a developed finite element model. Then, using micro-CNC and soft lithography, the microchip was constructed. Using the method presented in this study, 40-micron particles that represent the CTC clusters were separated from 2, 6 and 13 –micron particles, representing platelets, red blood cells and white blood cells respectively, from a sample of 5 ml with a flow rate of 1.5 ml/min
  9. Keywords:
  10. Microfluidic Chip ; Circulating Tumor Cells (CTC) ; Separation ; Inertial Focusing Devices

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