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Studying of Laccase Immobilization on Nanoparticles for Bisphenol A Removal

Sadeghzadeh, Sadegh | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51878 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Borghei, Mehdi; Ghobadi Nejad, Zahra
  7. Abstract:
  8. Bisphenol A is one of Endocrine Disturbing Chemicals. Enzymatic removal of bisphenol A is an interesting and green method. However, application of enzymes has been hampered due to lower operational stability and lots of difficulties in recovery and recycling. Enzyme immobilization can ba an innovative approach to circumvent these drawbacks. Laccase from Trametes hirsuta used for Bisphenol A removal. Firstly, magnetic nanoparticles were synthesized by coprecipitation method and aminofunctionalized by (3-aminopropyl)-mmethoxysilane. Subsequently, laccase was precipitated with i-propanol in the presence of aminofunctionalized nanoparticles and followed by addition of glutaraldehyde to the final concentration of 0.1% (v/v) as a crosslinking agent. The immobilized laccase was characterized by SEM and FTIR analysis. Activity recovery of 22.2% was achieved in Magnetic Cross-linked Enzyme Aggregates whileas, any immobilized laccase was not observed in Cross-linked Enzyme Aggregates due to poverty in lysine residues on the surface of laccase from Trametes hirsuta. In the following, Bisphenol A removal by utilizing the immobilized laccase was studied. It was obsereved that immobilized laccase has got better performance at 45℃ and pH=7. Finally, 83 % of Bisphenol A removal (with initial concentration of 55 ppm) was achieved in period of 9 hr
  9. Keywords:
  10. Bisphenol A ; Laccas Enzyme ; Magnetic Nanodot ; Trametes Hirsuta Mold ; Fixed Bed Reactor ; Enzyme Stabilization

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