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Design and Development of a Compound Skin Scaffold Capable of Drug Delivery for Skin Wound Healing

Bahadoran, Maedeh | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 51930 (08)
  4. University: Sharif University of Technology
  5. Department: Mechanical Engineering
  6. Advisor(s): Shamloo, Amir; Firoozbakhsh, Keykhosrow
  7. Abstract:
  8. Skin tissue as the first protective barrier to the body is always exposed to the most injuries. The purpose of this project is to design an optimum skin scaffold capable of drug delivery for skin wounds healing. To this end, the skin scaffold has been considered as a combination of a hydrogel structure containing polymer microspheres carrying growth factor. Polyvinyl alcohol and sodium alginate polymers were utilized to make the hydrogel substrate. The hydrogel structure was optimized from the viewpoint of the percentage of gelation, water absorption, porosity, mechanical properties, biodegradability, biocompatibility and considering the volume percent of utilized polymers and the manufacturing method as studied variables. Furthermore, the effect of gelatin presence was investigated on optimal hydrogel performance. Moreover, polycaprolactone microspheres were made using the double emulsion method and their structure and drug encapsulation efficiency were studied. The microspheres were then entrapped in the optimal hydrogel substrate. Subsequently, the drug release from three structures of hydrogel, polymer microspheres and compound scaffolds were studied and compared. In order to improve the antibacterial property of compound scaffold, mupirocin antibiotic was used in the hydrogel substrate. The physical, mechanical and biological performance of the compound scaffold were then investigated. Finally, the therapeutic performance of the compound scaffold was studied in the in-vivo condition. The results showed that the optimal volume percentage of the hydrogel substrate was 50:50. Also, the presence of gelatin improved the biological performance of the hydrogel. The hydrogel substrate containing microspheres was selected as the appropriate drug delivery approach due to its steady and controlled drug release. The proper scaffold antibacterial feature was also approved. The mechanical and biological studies of the compound scaffold revealed that the scaffold could be used to treat skin ulcers and repair damaged tissue. The in-vivo study also resulted in the proper performance of the compound scaffold compared to the commercial sample in the treatment of ulcers
  9. Keywords:
  10. Skin Scaffolds ; Skin Wounds ; Growth Factor ; Hydrogel ; Polymer Microspheres

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