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Pharmaceutical Active Compounds Removal by Immobilized Laccase on the Membrane

Golgoli, Mitra | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52081 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Borghei, Mehdi; Ghobadi Nejad, Zahra
  7. Abstract:
  8. Pharmaceutical active compounds existence in the water would cause serious ecological risks and human health-related adverse effects which turn to environmental concern, therefore several studies have done to remove pharmaceutical active compounds efficiently. Carbamazepine (CBZ), a widely used psychiatric drug, is one of the most frequently detected compounds in the surface water and groundwater which is studies in the current study. Recently, biocatalytic degradation using ligninolytic enzymes such as laccase provides a promising approach for their removal from water and wastewater. In this work, carbamazepine removal by immobilized laccase on modified membrane by multi wall carbon nanotube (MWCNT) has been studied. MWCNT would facilitate electron transfer between enzyme and substrate. Firstly, polyvinylidene fluoride (PVDF) membrane coated by MWCNT and PVDF/MWCNT nanocompoiste was made. Then, laccase from Trametes hirsute was covalently immobilized on the membranes. According to writer knowledge, laccase immobilization on PVDF/MWCNT nanocompoiste has not been studied. Activity recovery for immobilized laccase on the PVDF/MWCNT nanocomposite was 39% and for immobilized laccase on coated membrane was 30% which seems acceptable according to others studies. Also, thermal stability of immobilized laccase on PVDF/MWCNT retain 63% of its initial activity and coated membrane retained 52% of its initial activity after 2 hours in 70 °C and it was better than free lacasse which retaind 30% of its initial activity. Moreover, immobilized laccase on PVDF/MWCNT nanocomposite after 5 cycles and immobilized laccase on coated membrane after 3 cycles retained more than 20% of their initial activity. Finally, the membranes have been used for carbamazepine removal by initial 5 ppm concentration. Immobilized laccase on PVDF/MWCNT could remove 27% and immobilized laccase on coated membrane removed 23% after 48h. Carbamazepine adsorption by PVDF/MWCNT nanocomposite was negligible and for coated membrane, it was 14% which is owing to MWCNT on the membrane
  9. Keywords:
  10. Pharmaceutical Compound ; Carbamazpine ; Multi-Walled Carbon Nanotube ; Laccas Imobilization ; Lactase Enzyme

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