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Implementation of Paper Sheets into the Microfluidic Systems as an Appropriate Platform for Micro-Solid Phase Extraction

Hashemi Hedeshi, Mahin | 2019

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52155 (03)
  4. University: Sharif University of Technology
  5. Department: Chemistry
  6. Advisor(s): Bagheri, Habib
  7. Abstract:
  8. In this research, the surface of cellulose paper was modified with Hexadecyltrimethoxysilane, Phenyltriethoxysilane, (3-Aminopropyl)triethoxysilane and 3-(2,3-epoxypropoxy)propyltrimethoxysilane via chemical bonding. Afterwards, the prepared papers were placed in the microfluidic device as sorbent in a sandwiched format. A syringe pump was used to drive the sample into the microfluidic channel. In order to investigated the performance of these sorbents, two types of anlaytes including toxins and antidepressant drugs were chosen as target analytes. In the case of toxins, ethion, fenthion, profenfose, diazinon, atrazine and ametryn were studied. among the antidepressant drugs, amitripline,clomipramine,trimipramine and citalopram were selected for further investigation. In order to better evaluate the obtained data, some statistical methods, including PCA and HCA were used. After comparing the capacity of sorbents with each other by means of statistical methods, the surface modified paper by triethoxysilane, was selected as higher capacity sorbent with respect to other modified papers. Afterwards, the influential parameters on extraction process such as sample dilution factor, acidity, sample flow rate, number of sampling cycles, type of desorbing solvent and its volume were optimized. In order to confirm the proposed method, gas chromatography was eventually utilized. The detection and quantification limits of the method were in the ranges of 0.005-0.01 µg L-1 and 0.02-0.04 µg L-1, respectively. The interday and intraday reproducibility (RSD%) were lower than 11% (n=3). The calibration graph for amitriptyline, trimipramine and clomipramine were linear in range of 6-200 µg L-1 . The proposed sorbent was used for the analysis of biological sample (urine). The relative recovery percentage in the range of 95-103 was acquired
  9. Keywords:
  10. Paper Based Extractive Phase ; Gas Chromatography ; Microfluidic Devices ; Solid Phase Extraction

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