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An ultrasensitive label free human papilloma virus DNA biosensor using gold nanotubes based on nanoporous polycarbonate in electrical alignment

Shariati, M ; Sharif University of Technology | 2019

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  1. Type of Document: Article
  2. DOI: 10.1016/j.aca.2018.09.062
  3. Publisher: Elsevier B.V , 2019
  4. Abstract:
  5. An impedimetric human papilloma virus (HPV) DNA biosensor based on gold nanotubes (AuNTs) in label free detection was materialized. The AuNTs decorated nanoporous polycarbonate (AuNTs-PC) template as biosensor electrode was fabricated by electrodeposition method. The single strand DNA (ss-DNA) probe was covalently immobilized onto the AuNTs-PC electrode. The hybridization of target sequences with the ss-DNA probe was observed by the electrochemical impedance spectroscopy (EIS). The biosensor showed high selectivity and could differentiate between the complementary, mismatch and non-complementary DNA sequences. The EIS measurements were matched to Randle's equivalent circuit. The negatively-charged HPV DNA oligonucleotides under external electric field were oriented in a preferred direction and the bio-sensing responses were intensified by controlling the immobilization and hybridization of the sequences on the AuNTs surface. The fabricated DNA biosensor under electric field amplification was stable up to six weeks and demonstrated 97% of its initial detection responses. The biosensor displayed the HPV DNA hybridization detection in very low concentrations in the linear response ranges of 0.01 pM–1 μM and was able to acquire a limit of detection (LOD) of 1 fM. © 2018 Elsevier B.V
  6. Keywords:
  7. Biosensor ; Electrochemical impedance spectroscopy ; Gold nanotubes ; Human papilloma virus DNA ; Nanoporous polycarbonate ; Biosensors ; DNA ; Electric fields ; Equivalent circuits ; Nanotubes ; Oligonucleotides ; Polycarbonates ; Probes ; Spectroscopy ; Tumors ; Viruses ; Yarn ; DNA hybridization detection ; Electric field amplification ; Electrodeposition methods ; External electric field ; Human papilloma virus ; Nano-porous ; Single strand DNA (ss-DNA) ; DNA sequences ; Gold nanotube ; Nanotube ; Oligonucleotide ; Polycarbonate ; Single stranded DNA ; Unclassified drug ; Virus DNA ; Base mispairing ; Controlled study ; DNA hybridization ; DNA sequence ; Electric field ; Genetic procedures ; Immobilization ; Impedance spectroscopy ; Nonhuman ; Priority journal ; Wart virus ; Chemistry ; Electrochemical analysis ; Electrode ; Human papillomavirus type 16 ; Isolation and purification ; Porosity ; Procedures ; Reproducibility ; Biosensing Techniques ; Electrochemical Techniques ; Electrodes ; Gold ; Human papillomavirus 16 ; Limit of Detection ; Polycarboxylate Cement ; Porosity ; Reproducibility of Results
  8. Source: Analytica Chimica Acta ; Volume 1048 , 2019 , Pages 31-41 ; 00032670 (ISSN)
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0003267018311668