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Optimal condition for optical trapping of large particles: Tuning the laser power and numerical aperture of the objective
Gorjizadeh Alinezhad, H ; Sharif University of Technology | 2019
436
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- Type of Document: Article
- DOI: 10.1364/JOSAB.36.003053
- Publisher: OSA - The Optical Society , 2019
- Abstract:
- A Gaussian laser beam tightly focused through a high-numerical-aperture objective lens, so-called optical tweezers, is widely used for piconewton-range force spectroscopy. Utilizing a proper value for parameters such as bead size, numerical aperture of the objective, and power of the laser is always a challenge. Here, we show which set of values for the parameters can maximize lateral trapping efficiency. Our results show that for a high-numerical-aperture force spectroscopy, a bead with a diameter of 4–5 µm is suitable, and that for manipulation using large beads, utilizing a proper value for laser power and numerical aperture of the objective is crucial. We present a practical method for choosing the power of the laser that maximizes lateral trapping efficiency. © 2019 Optical Society of America
- Keywords:
- Efficiency ; Laser beams ; Laser optics ; Lenses ; Force spectroscopy ; Gaussian laser beam ; High numerical aperture objective ; High numerical apertures ; Numerical aperture ; Optimal conditions ; Practical method ; Trapping efficiencies ; Optical tweezers
- Source: Journal of the Optical Society of America B: Optical Physics ; Volume 36, Issue 11 , 2019 , Pages 3053-3059 ; 07403224 (ISSN)
- URL: https://www.osapublishing.org/josab/abstract.cfm?uri=josab-36-11-3053