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- Type of Document: M.Sc. Thesis
- Language: Farsi
- Document No: 52757 (06)
- University: Sharif University of Technology
- Department: Chemical and Petroleum Engineering
- Advisor(s): Roosta Azad, Reza; Tahmasebpoor, Maryam
- Abstract:
- Almost all of the country's industrial enzyme needs are met through imports. In this regard, much attention has been paid to industrial production of detergent enzymes such as protease, lipase, amylase and cellulase. Proteases, are the most important industrial enzymes with 60% of the world's enzyme market. Extracellular alkaline proteases of microbial origin are most widely used in the laundry detergent industry to help clear protein stains on clothing caused by blood, milk, eggs, and meat. In this study, bacterial alkaline protease enzyme produced by Bacillus subtilis ATCC6633 was used as a model of functional enzymes in detergent industries.Enzyme immobilization-drying operations are one of the most important downstream processes in the production of a biological enzyme product which results in increased stability and activity of the enzyme in the form of granules. The aim of this study is to obtain stable alkaline protease enzyme liquid and its immobilization on solid substrate as powders with minimum activity loss to use in detergent industries. In the present study, the activity stability analysis and staining of the enzyme power in the wash conditions was investigated.For this purpose, produced liquid alkali protease enzyme, was completely stabilized for one year of storage at 4 ° C by the addition of a binary stabilizer containing CaCl2 and boric acid with optimum composition of 0.1 and 2 % w/v as protective agents. The stabilized enzyme was fixed by applying a rotary system with spraying on a solid bed of sodium sulfate and FCC with a 70% to 30% composition and then dried in a bed dryer. By selecting the FCC carrier, the challenge of agglomeration of the granules was also solved during the stabilization-drying operation. Comparison of the protease activity values of the commercial sample of Novozymes Company and the produced granule sample show that the estimated difference between the two samples is approximately 80-85 times, and this difference was confirmed by the real-time performance test of water washing in Tehran. Obviously, this difference can be reduced by strain engineering, culture medium optimization and concentration methods improvement
- Keywords:
- Stability ; Drying ; Agglomeration ; Alkaline Protease Enzyme ; Enzyme Stabilization ; Protective Agent
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