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Development of a Polymeric Scaffold for Periodontal Regeneration

Rahimi Nasrabadi, Kosar | 2020

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  1. Type of Document: M.Sc. Thesis
  2. Language: Farsi
  3. Document No: 52970 (06)
  4. University: Sharif University of Technology
  5. Department: Chemical and Petroleum Engineering
  6. Advisor(s): Abdekhodaie, Mohammad Jafar
  7. Abstract:
  8. Periodontitis is a common inflammatory disease that affects the periodontium.Periodontium includes two hard tissues of cementum and alveolar bone and also soft tissue of periodontal ligament. The appropriate function is based on the consistency and accurate interaction of them. The complex structure, the low potential of the body for spontaneous healing, and technical problems such as bacteria accumulation, limited access, and small operating field cause no complete treatment can be achieved until now.In this project, at first collagen type I was extracted from Bovine Achilles tendon. Then, polymer modification was done to 39.95 µg/mg (Tyramine/ Collagen). An In situ gel based on modified collagen in the presence of H2O2 and horseradish peroxidase (HRP) was developed. This scaffold characterized in terms of gelation time, swelling ratio, morphology, rheological analysis, and degradation time. The gelation of the optimum sample took place within a minute. The average pore size for that hydrogel was 77 µm. The rheological analysis showed that the storage modulus was 6890 Pa. The optimum sample was entirely degraded after 20 days. The optimum sample was compared with in situ gel based on collagen type I from rat tail. It was shown that the physical properties of hydrogel based on Bovine Achilles tendon were more appropriate for periodontal regeneration. After that, platelet lysate was successfully incorporated into hydrogel as a source of growth factors and anti-inflammatory cytokines. The effect of platelet lysate on the physical properties was evaluated.The average pore size of the hydrogel incorporated with platelet lysate was 103 µm. The storage modulus of that was 5680 Pa, and the degradation time was 15 days. In vitro release behavior of growth factors was investigated by using lysozyme as a model protein. Lysozyme was released to four days. In the end, metronidazole as an attractive antimicrobial agent was loaded into the scaffold, and the drug release studied in vitro. This study showed drug release for the first hours. It is expected this scaffold facilitates the periodontal wound healing process as well as overcomes some technical challenges due to being formed localized in deep periodontal pockets.

  9. Keywords:
  10. Platelet Lysate ; Metronidasole ; In-Situ Forming Hydrogel ; Collagen Type I ; Periodontal Tissue Regeneration ; Tooth Diseases

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