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MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation

Shahriari, F ; Sharif University of Technology | 2020

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  1. Type of Document: Article
  2. DOI: 10.1016/j.exer.2019.107883
  3. Publisher: Academic Press , 2020
  4. Abstract:
  5. Retinal pigment epithelial (RPE) cells are indispensable for eye organogenesis and vision. To realize the therapeutic potential of in vitro-generated RPE cells for cell-replacement therapy of RPE-related retinopathies, molecular mechanisms of RPE specification and maturation need to be investigated. So far, many attempts have been made to decipher the regulatory networks involved in the differentiation of human pluripotent stem cells into RPE cells. Here, we exploited a highly-efficient RPE differentiation protocol to determine global expression patterns of microRNAs (miRNAs) during human embryonic stem cell (hESC) differentiation into RPE using small RNA sequencing. Our results revealed a significant downregulation of pluripotency-associated miRNAs along with a significant upregulation of RPE-associated miRNAs in differentiating cells. Our functional analyses indicated that two RPE-enriched miRNAs (i.e. miR-125b and let-7a) could promote RPE fate at the expense of neural fate during RPE differentiation. Taken together, these mechanistic interrogations might shed light on a better understanding of RPE cell development and provide insights for the future application of these cells in regenerative medicine. © 2019 Elsevier Ltd
  6. Keywords:
  7. Embryonic stem cells ; Let-7 ; MicroRNA ; MiR-125b ; Retinal pigment epithelium ; Small RNA sequencing ; Let 7a ; MicroRNA 125b ; RNA precursor ; Unclassified drug ; MIRN125 microRNA, human ; Mirnlet7 microRNA, human ; Cell maturation ; Gene expression ; Human cell ; Human cell culture ; Human embryonic stem cell ; Priority journal ; Receptor down regulation ; Retinal pigment epithelium ; RNA sequence ; Upregulation ; Cytology ; Fluorescence microscopy ; Genetics ; Metabolism ; Physiology ; Real time polymerase chain reaction ; Signal transduction ; Transmission electron microscopy ; Cell Differentiation ; Cell Line ; Flow Cytometry ; Gene Expression Profiling ; Human Embryonic Stem Cells ; Immunohistochemistry ; MicroRNAs ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Phagocytosis ; Real-Time Polymerase Chain Reaction
  8. Source: Experimental Eye Research ; Volume 190 , 2020
  9. URL: https://www.sciencedirect.com/science/article/abs/pii/S0014483518307772